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Recombinant Human Angiopoietin-like 1 Flag-tag Protein, CF

Catalog # 10013-AN | R&D Systems, Inc. a Bio-Techne Brand
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10013-AN-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked oligomer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Angiopoietin-like Protein 1/ANGPTL1 protein
Gly24-Asp491, with a C-terminal Flag-tag

Purity

>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg22

Predicted Molecular Mass

56 kDa

SDS-PAGE

Predominant band at 60 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Integrin alpha 1 beta 1 Recombinant Human Integrin  alpha1 beta1 (Catalog # 7064-AB) is used at 1 μg/mL, Recombinant Human Angiopoietin‑like Protein 1/ANGPTL1 binds with an ED50 of 8-80 ng/mL.

Scientific Data Images for Recombinant Human Angiopoietin-like 1 Flag-tag Protein, CF

Recombinant Human Angiopoietin-like 1 Flag-tag Protein Binding Activity

Recombinant Human Angiopoietin-like 1 Flag-tag Protein Binding Activity

When Recombinant Human Integrin a1 beta1 (Catalog # 7064-AB) is used at 1 µg/mL, Recombinant Human Angiopoietin-like Protein 1/ANGPTL1 Flag-tag (Catalog # 10013-AN) binds with an ED50 of 8-80 ng/mL.
Recombinant Human Angiopoietin-like 1 Flag-tag Protein SDS-PAGE

Recombinant Human Angiopoietin-like 1 Flag-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Angiopoietin‑like Protein 1/ANGPTL1 Flag-tag (Catalog# 10013-AN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a predominant band at 60 kDa and 180 kDa, respectively.

Formulation, Preparation and Storage

10013-AN
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CHAPS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 1/ANGPTL1

Angiopoietin like Protein 1 (ANGPTL1), also known as Angioarrestin, ARP1 and ANG3, is a secreted glycoprotein that contains a coiled coil domain at the N-terminus and a fibrinogen like domain at the C-terminus (1). ANGPTL1 is one of the seven members of the Angiopoietin-like family proteins. Similar to angiopoietins, ANGPTL1 secretes as a disulfide linked homo-oligomer (2-4). Mature human ANGPTL1 shares 94% sequence identity with its mouse homolog. ANGPTL1 is highly expressed in adrenal gland, placenta, thyroid gland, heart, skeletal muscle and small intestine, and weakly expressed in testis, ovary, colon, pancreas, kidney and stomach (1). Expression of ANGPTL1 is down-regulated in various cancers (4-6). ANGPTL1 has anti-angiogenic functions, inhibiting endothelial cell proliferation, migration, tube formation, and adhesion (4, 5). ANGPTL1 is a tumor suppressor. Over-expression of ANGPTL1 inhibits tumor growth (4, 5), migration, and metastasis (6, 7). Upon binding to integrin alpha1/ beta1, the FAK/Src-JAK-STAT3 signaling pathway is inhibited in hepatocellular carcinoma, promoting apoptosis and suppressing cancer invasion and angiogenesis (6). Binding to integrin alpha1/ beta1 also leads to the inhibition of FAK/ERK/SP1 signaling pathway to induce the expression of micro RNA 630, which suppresses the zinc finger protein SLUG to inhibit lung cancer cell mobility (7). ANGPT-L1 has been shown to directly interact with and antagonize MET receptor activity in hepatocellular carcinoma (8).

References

  1. Kim, I. et al. (1999), FEBS Lett 443:353
  2. Kim, K-T. et al. (2005) J. Biol. Chem. 280:20126.
  3. Procopio, W. N., et al. (1999) J. Biol. Chem. 274:30196
  4. Dhanabal, M. et al. (2002) Cancer Res 62:3834.
  5. Smagur, A. et al. (2005) Acta Biochim Pol. 52:875.
  6. Yan, Q. et al. (2017) Cancer Res. 77:5831.
  7. Kuo, T-C. et al. (2013) J Clin Invest 123:1082.
  8. Chen H-A, et al. (2016) Hematology 64(5):1637.

Alternate Names

Angiopoietin like Protein 1, ANGPTL1, AngY1, ARP1

Entrez Gene IDs

9068 (Human); 72713 (Mouse); 679942 (Rat)

Gene Symbol

ANGPTL1

UniProt

Additional Angiopoietin-like Protein 1/ANGPTL1 Products

Product Documents for Recombinant Human Angiopoietin-like 1 Flag-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Angiopoietin-like 1 Flag-tag Protein, CF

For research use only

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