Recombinant Human Alkaline Phosphatase/ALPL Protein, CF

Name: Recombinant Human Alkaline Phosphatase/ALPL Protein, CF
Brand: R&D Systems
SKU: 2909-AP
Price: 351 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 2909-AP

Citations (3)

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Key Product Details

Source:	NS0
Accession #:	P05186
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human Alkaline Phosphatase/ALPL Protein, CF

Product Specifications for Recombinant Human Alkaline Phosphatase/ALPL Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Alkaline Phosphatase/ALPL protein Leu18-Ser502, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu18
Predicted Molecular Mass	54 kDa
SDS-PAGE	65-75 kDa, reducing conditions
Activity	Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >30,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2909-AP

Formulation	Supplied as a 0.2 μm filtered solution in HEPES, NaCl, MgCl₂ and ZnCl₂.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: Alkaline Phosphatase/ALPL

Four distinct genes encode alkaline phosphatases (APs) in humans (1). The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue‑nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers (2). There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 503 to 524) is not present in the mature form.

Item	Value
References	Le Du, M.-H. and J.L. Millan (2002) J. Biol. Chem. 277:49808. Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1. Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.
Long Name	Alkaline Phosphatase Liver
Entrez Gene IDs	249 (Human); 11647 (Mouse)
Alternate Names	ALPL, AP-TNAP, APTNAP, Akp2, Alkaline phosphatase liver/bone/kidney isozyme, EC 3.1.3.1, FLJ40094, FLJ93059, HOPS, MGC161443, TNAP, TNSALP, TNSALPMGC167935, alkaline phosphatase, liver/bone/kidney, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphomonoesterase, glycerophosphatase, liver/bone/kidney-type alkaline phosphatase, tissue-nonspecific ALP

Citations for Recombinant Human Alkaline Phosphatase/ALPL Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Alkaline Phosphatase/ALPL Protein, CF include:

Species: Human
Sample Types: Small Molecule
Applications: Bioassay

CL Pervan-Ste et al. (2022-01-01), Ocular Hypotensive Properties and Biochemical Profile of QLS-101, a Novel ATP-Sensitive Potassium (KATP) Channel Opening Prodrug Investigative Ophthalmology Visual Science, 2022;63(4):26.
PMID: 35486069
Species: Mouse
Sample Types: Cell Lysates
Applications: Bioassay

Y Sun et al. (2021-01-01), Mitochondrial TNAP controls thermogenesis by hydrolysis of phosphocreatine Nature, 0(0).
PMID: 33981039
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay

M Pettengill et al. (2017-01-01), Human alkaline phosphatase dephosphorylates microbial products and is elevated in preterm neonates with a history of late-onset sepsis PLoS ONE, 12(4):e0175936.
PMID: 28448526

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