Recombinant Human Agrin Protein, CF

Name: Recombinant Human Agrin Protein, CF
Brand: R&D Systems
SKU: 6624-AG

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 6624-AG

Citations (6)

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Source:	CHO
Accession #:	NP_940978
Structure / Form:	Monomer
Applications:	Bioactivity

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Product Specifications for Recombinant Human Agrin Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived human Agrin protein Ala1260-Pro2045, with an N-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	His
Predicted Molecular Mass	83.5 kDa
SDS-PAGE	100-110 kDa, reducing conditions
Activity	Measured by the ability of the immobilized protein to support the adhesion of U‑87 MG human glioblastoma/astrocytoma cells. The typical ED₅₀ for this effect is 0.9-3.6 μg/mL after 1 hour incubation at 37 °C. Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6624-AG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 300 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Agrin

Agrin is a 400‑600 kDa heparan sulfate proteoglycan component of the extracellular matrix. The N-terminal half of human Agrin, which mediates ECM interactions, contains a Laminin-binding NtA domain, nine Kazal-type protease inhibitor domains, two Laminin EGF-like domains, and one SEA domain. The C‑terminal half contains four EGF-like repeats and three Laminin globular G domains. Rat Agrin lacks the NtA domain, and mouse and chick Agrin include the NtA domain only by the use of an alternate promoter. Additional isoforms are generated by alternate splicing at sites Y and Z in the C‑terminal half of rat Agrin (known as A and B, respectively in chick). Agrin isoforms that contain an insert at site Z (Z+ forms) are known as neural Agrin and are selectively produced by motoneurons. Other isoforms are known as muscle Agrin and are additionally expressed in non-neuronal tissues, particularly in basement membranes of the lung and kidney (1-3). This recombinant protein consists of the C‑terminal half of human Agrin corresponding to the Z- isoform of rat Agrin. It shares 60%, 78%, and 80% amino acid sequence identity with comparable regions of chick, mouse, and rat Agrin, respectively. The C‑terminal half of Z- and Z+ Agrin binds to alpha ‑Dystroglycan and mediates adhesion between motoneurons and myotubes at the neuromuscular junction (NMJ) (4-6). In contrast, only Z+ Agrin is effective at inducing clustering of the postsynaptic Acetylcholine Receptor (AChR) and presynaptic motoneuron differentiation (7, 8). Agrin-induced AChR clustering requires a myotube receptor complex that contains alpha ‑Dystroglycan, MuSK, and LRP4 (4, 9-11). Agrin exhibits many functions in addition to NMJ development. It is enriched in senile Alzheimer's disease plaques where it binds the A beta (1‑40) peptide and promotes amyloid fibril formation (12). It regulates neuronal excitability by binding and inhibiting the alpha 3 subunit of the neuronal Na/K ATPase (13). It functions as an epithelial cell attachment receptor for HIV-1 through interactions with the gp41 coat protein (14). During T cell activation, Agrin contributes to formation of the immunological synapse and regulates the threshold of T cell activation (15).

Item	Value
References	Jury, E.C. and P.S. Kabouridis (2010) Arthritis Res. Ther. 12:205. Bezakova, G. and M.A. Ruegg (2003) Nat. Rev. Mol. Cell Biol. 4:295. Groffen, A.J.A. et al. (1998) Eur. J. Biochem. 254:123. Gee, S.H. et al. (1994) Cell 77:675. Sugiyama, J. et al. (1994) Neuron 13:103. Gesemann, M. et al. (1998) J. Biol. Chem. 273:600. Burgess, R.W. et al. (1999) Neuron 23:33. Ferns, M.J. et al. (1993) Neuron 11:491. Glass, D.J. et al. (1996) Cell 85:513. Kim, N. et al. (2008) Cell 135:334. Zhang, B. et al. (2008) Neuron 60:285. Cotman, S.L. et al. (2000) Mol. Cell. Neurosci. 15:183. Hilgenberg, L.G.W. et al. (2006) Cell 125:359. Alfsen, A. et al. (2005) Mol. Biol. Cell 16:4267. Khan, A.A. et al. (2001) Science 292:1681.
Entrez Gene IDs	375790 (Human); 11603 (Mouse); 25592 (Rat)
Alternate Names	AGRN, Agrin, agrin proteoglycan

Citations for Recombinant Human Agrin Protein, CF (6)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Agrin Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

M Pandya et al. (2022), The Hippo Pathway Effectors YAP/TAZ Are Essential for Mineralized Tissue Homeostasis in the Alveolar Bone/Periodontal Complex Journal of developmental biology, 10(1).
PMID: 35323233
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Stoklund D et al. (2021), Human motor units in microfluidic devices are impaired by FUS mutations and improved by HDAC6 inhibition Stem Cell Reports, 0(0).
PMID: 33891869
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

CY Lin et al. (2019), iPSC-derived functional human neuromuscular junctions model the pathophysiology of neuromuscular diseases JCI Insight, 4(18).
PMID: 31534050
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

ME Katt et al. (2018), Functional brain-specific microvessels from iPSC-derived human brain microvascular endothelial cells: the role of matrix composition on monolayer formation Fluids Barriers CNS, 15(1):7.
PMID: 29463314
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

N Nagy et al. (2018), Collagen 18 and agrin are secreted by enteric neural crest cells to remodel their microenvironment and regulate their migration during ENS development Development, 0(0).
PMID: 29678817
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), The Different Binding Properties of Cultured Human Corneal Endothelial Cell Subpopulations to Descemet's Membrane Components Invest Ophthalmol Vis Sci, 57(11):4599-605.
PMID: 27598865

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Showing 1 - 6 of 6 citations Showing All

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Recombinant Human Agrin Protein, CF

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