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Recombinant Human Adiponectin/Acrp30 Protein, CF Best Seller

Bio-Techne includes R&D Systems | Catalog # 1065-AP

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1065-AP-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Oligomer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Adiponectin/Acrp30 protein
Glu19-Asn244, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu19

Predicted Molecular Mass

25.4 kDa

SDS-PAGE

27-35 kDa, reducing conditions

Activity

Measured by its ability to induce TIMP-1 secretion by mouse macrophages. Kumada, M. et al. (2004) Circulation 109:2046.
The ED50 for this effect is 1.20-18.0 µg/mL.

Formulation, Preparation and Storage

1065-AP
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature human Adiponectin consists of a 60 amino acid (aa) N‑terminal collagenous region and a 137 aa C‑terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (10-12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).

References

  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
  9. Waki, H. et al. (2005) Endocrinology 146:790.
  10. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  11. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  12. Wang, Y. et al. (2008) Biochem. J. 409:623.
  13. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  14. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Entrez Gene IDs

9370 (Human); 11450 (Mouse); 246253 (Rat)

Gene Symbol

ADIPOQ

UniProt

Additional Adiponectin/Acrp30 Products

Product Documents for Recombinant Human Adiponectin/Acrp30 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Adiponectin/Acrp30 Protein, CF

For research use only

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