Product Specifications for Recombinant Human ADAMTS-L2 His-tag Protein, CF
Chinese Hamster Ovary cell line, CHO-derived human ADAMTSL2 protein Asp23-Ser951 with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
130-144 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Human ADAMTSL2 His-tag (Catalog# 10950-AD) immobilized at 2.00 μg/mL, 100 μL/well, the concentration of Recombinant Human Lysyl Oxidase Homolog 2 Protein (Catalog # 2639-AO) that produces 50% of the optimal binding response is approximately 0.60-3.00 μg/mL.
Scientific Data Examples for Recombinant Human ADAMTS-L2 His-tag Protein, CF
Recombinant Human ADAMTS-L2 His-tag Protein Binding Activity.
When Recombinant Human ADAMTSL2 His-tag (Catalog# 10950-AD) is immobilized at 2.00 μg/mL, 100 μL/well, the concentration of Recombinant Human Lysyl Oxidase Homolog 2 Protein (2639-AO) that produces 50% of the optimal binding response is approximately 0.60-3.00 μg/mL.
Recombinant Human ADAMTS-L2 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human ADAMTS-L2 His-tag Protein (Catalog # 10950-AD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 130-144 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with Trehalose.
Reconstitute at 100 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
ADAMTSL2 (a disintegrin and metalloproteinase with thrombospondin motifs like 2), is one protein from a seven-member family of ADAMTS-like secreted proteins that are related to ADAMTS zinc metalloproteases but do not contain a metalloprotease domain. ADAMTSL2 contains a multidomain structure that includes an N-terminal signal sequence, C-terminal thrombospondin repeats, ADAMTS cysteine-rich and spacer modules, a PLAC domain and an N-glycan rich module unique amongst ADAMTSL family members (1). These domains play a substantial role in ADAMTS specificity and ADAMTSLs are also expected to have specific extracellular ligands. ADAMTSL2 is abundantly expressed in liver, lung, and spleen and resides in the extracellular matrix (ECM) (2). It has been shown to interact with many ECM proteins including fibrillin microfibrils (3), members of the lysyl oxidase family such as LOXL2 (4), and latent transforming growth factor beta binding protein 1 (LTBP1) (5). The interaction between ADAMTSL2 and LTBP1 and fibrillin indicates ADAMTSL2 may have a role in modulation of microfibrillar formation and function (3,5) and ADAMTSL2 has been found upregulated in cardiac fibrosis (6). Through its interaction with the LTBP1 protein and lysyl oxidase family members, ADAMTSL2 protein has been suggested to regulate the availability of TGF-beta and consequent TGF-beta signaling (1,5). Several mutations of ADAMTSL2 lead to a heterogeneous autosomal recessive disease, Geleophysic dysplasia 1 (7, 8). Geleophysic dysplasia 1 may be specifically caused by impaired secretion of ADAMTSL2 due to glycosylation differences (9). A variant of ADAMTSL2 has also been shown to result in an autosomal dominant inherited connective tissue disorder consistent with Ehlers-Danlos syndrome (10).
Mead, T.J. and S.S. Apte. (2018) Matrix Biol. 71:225.
Koo, B. et al. (2007) Matrix Biol. 26:431.
Hubmacher, D. and S. S. Apte. (2015) Matrix Biol. 47:34.
Aviram, R. et al. (2019) Matrix Biol. 75-76:114-125.
Le Goff, C. et al. (2008) Nat. Genet. 9:1119-23.
Trenson, S. et al. (2021) Circ. Heart Fail. 14:e006979.
Allali, S. et al. (2011) J. Med. Genet. 48:417.
Marzin, P. et al. (2009) Gene Reviews. Internet:1993-2020.
Zhang, A. et al. (2020) J. Biol. Chem. 295:15742.
Steinle, J. et al. (2021) Am. J. Med. Genet. A. 185:743.
ADAMTS-like Protein 2
Entrez Gene IDs
ADAMTSL2, ADAMTSL2 ADAMTS-like 2 , GPHYSD1
Citations for Recombinant Human ADAMTS-L2 His-tag Protein, CF
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Product Documents for Recombinant Human ADAMTS-L2 His-tag Protein, CF
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