Recombinant Human ADAM8 Protein, CF

Name: Recombinant Human ADAM8 Protein, CF
Brand: R&D Systems
SKU: 1031-AD
Rating: 4 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1031-AD

Citations (4)

(2)

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Key Product Details

Source:	NS0
Accession #:	P78325
Applications:	Enzyme Activity

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Product Specifications for Recombinant Human ADAM8 Protein, CF

Source	Mouse myeloma cell line, NS0-derived human ADAM8 protein Met1-Pro497, with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu158
Predicted Molecular Mass	38 kDa
SDS-PAGE	42 kDa, reducing conditions
Activity	Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH₂ (Catalog # ES003). The specific activity is >1 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1031-AD

Formulation	Supplied as a 0.2 μm filtered solution in Glycerol, Tris, NaCl and CaCl₂.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

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Background: ADAM8

ADAM8, also known as cell surface antigen MS2 or CD156a, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). ADAM8 can cleave a variety of substrates and has been shown as a sheddase for the low affinity IgE receptor CD23 and the neural recognition molecule CHL1 (3, 4). Expression and regulation studies suggest that ADAM8 is a novel osteoclast stimulating factor and may play a role in asthma (5, 6). The 824 amino acid precursor of human ADAM8 consists of a signal peptide (residues 1 to 16), a pro peptide (residues 17 to 199), a metaloprotease domain (residues 200 to 400), a disintegrin-like domain (residues 408 to 494), a cysteine-rich region (residues 497 to 613), an EGF-like domain (residues 614 to 640), a transmembrane region (residues 656 to 676) and a cytoplasmic domain (residues 677 to 824). The purified rhADAM8 (residues 158 to 497) contains a part of the pro peptide and the entire metalloprotease and disintegrin-like domains. It can be activated and assayed under the conditions described in the Activity Assay Protocol.

Item	Value
References	Yoshiyama, K. et al. (1997) Genomics 41:56. Moss, M.L. and J.W. Bartsch (2004) Biochemistry 43:7227. Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469. Naus, S. et al. (2004) J. Biol. Chem. 279:16083. Choi, S.J. et al. (2001) J. Bone Miner. Res. 16:814. King, N.E. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:257.
Long Name	A Disintegrin and Metalloprotease-like Domain 8
Entrez Gene IDs	101 (Human)
Alternate Names	ADAM 8, ADAM metallopeptidase domain 8, ADAM8, CD156a, CD156a antigen, CD156human leukocyte differentiation antigen, Cell surface antigen MS2, EC 3.4.24, EC 3.4.24.-, MGC134985, MS2, MS2disintegrin and metalloproteinase domain-containing protein 8, a disintegrin and metalloproteinase domain 8

Citations for Recombinant Human ADAM8 Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human ADAM8 Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MW Johansson et al. (2017), IL-5-stimulated eosinophils adherent to periostin undergo stereotypic morphological changes and ADAM8-dependent migration Clin. Exp. Allergy, 0(0).
PMID: 28378503
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Schlomann U et al. (2015), ADAM8 as a drug target in pancreatic cancer. Nat Commun, 6(0):6175.
PMID: 25629724
Species: Bacteria - E. Coli
Sample Types: Cell Lysates
Applications: Enzyme Assay

McDonald A et al. (2014), A new paradigm for enzymatic control of alpha-cleavage and beta-cleavage of the prion protein. J Biol Chem, 289(2):803-13.
PMID: 24247244
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gomez-Gaviro M et al. (2007), Expression and regulation of the metalloproteinase ADAM-8 during human neutrophil pathophysiological activation and its catalytic activity on L-selectin shedding. J. Immunol., 178(12):8053-63.
PMID: 17548643

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Customer Reviews for Recombinant Human ADAM8 Protein, CF (2)

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Recombinant Human ADAM8 Protein, CF

Name: Youwen Zhang

Application: Enzymatic activity in vitro

Verified Customer | Posted 04/17/2020
Recombinant Human ADAM8 Protein, CF

Name: Anonymous

Application: Enzymatic activity in vitro

Verified Customer | Posted 05/07/2017

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Product Documents for Recombinant Human ADAM8 Protein, CF

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Recombinant Human ADAM8 Protein, CF

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Product Specifications for Recombinant Human ADAM8 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: ADAM8

Citations for Recombinant Human ADAM8 Protein, CF (4)

Customer Reviews for Recombinant Human ADAM8 Protein, CF (2)

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Recombinant Human ADAM8 Protein, CF

Recombinant Human ADAM8 Protein, CF

Product Documents for Recombinant Human ADAM8 Protein, CF

Product Datasheets

COA

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