Recombinant Human Activin AB Protein

Name: Recombinant Human Activin AB Protein
Brand: R&D Systems
SKU: 1066-AB
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1066-AB

Citations (5)

(1)

Product Datasheet / COA / SDS

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Key Product Details

Source:	CHO
Accession #:	NP_002183 ( beta A) & NP_002184 ( beta B)
Structure / Form:	Disulfide-linked heterodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human Activin AB Protein

Source

Chinese Hamster Ovary cell line, CHO-derived human Activin AB protein

Human Activin beta A
(Gly311 - Ser426)
Accession # NP_002183.1

Human Activin beta B (Gly293 - Ala407) Accession # NP_002184.2
N-terminus	C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.1 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gly311( beta A subunit) & Gly293 ( beta B subunit)

Predicted Molecular Mass

12.8 kDa ( beta A subunit) and 13 kDa ( beta B subunit)

SDS-PAGE

14 kDa, reducing conditions

Activity

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED₅₀ for this effect is 0.2‑1.2 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1066-AB

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1066-AB/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Reconstitution	Reconstitute at 50 µg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Activin AB

Activins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins that were originally purified from gonadal fluids as proteins that stimulated pituitary follicle stimulating hormone (FSH) release. Activin proteins have since been shown to have a wide range of biological activities including: mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and roles in reproductive physiology. Activins are produced as precursor proteins with an amino-terminal propeptide that is cleaved to release the carboxy-terminal bioactive ligands. Activins are homodimers or heterodimers of the various beta subunit isoforms. Five beta subunits (mammalian beta _A, beta _B, beta _C, beta _E and Xenopus beta _D) have been cloned. The nomenclature reflects the subunit composition of the proteins: Activin A ( beta _A - beta _A), Activin B ( beta _B - beta _B), and Activin AB ( beta _A - beta _B). Activin A, Activin B, and Activin AB are present in gonadal tissues and are biologically active proteins. However, little is known about the contribution of the other beta subunits to Activin formation and function since knock-outs of beta _C and beta _E in mice do not exhibit a phenotype.

At the amino acid sequence level, the mature human beta _A subunit is 100% identical to mouse beta _A, while the mature human and mouse beta _B subunits share 98% identity. The mature beta _A and beta _B subunits share less than 80% amino acid identity. Mice with targeted mutations of beta _A, beta _B, or both genes do not show mesodermal defects, indicating Activin is not involved in mesoderm formation in mammals as it is in Xenopus. Also, the double homozygous mutants have the whole spectrum of defects associated with either mutation alone, suggesting that Activin beta _A and beta _B do not compensate for one another, nor do they have overlapping functions. Similar to other TGF-beta family members, Activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type II. Activin binds directly to ACT RII, the complex then associates with ACT RI and initiates signaling through the SMADs.

Item	Value
References	Woodruff, T.K. (1998) Biochemical Pharmacology 55:953. Ying, S.Y. et al. (1997) Proc. Soc. Exp. Biol. Med. 214:114. Chang, H. et al. (2001) Mol. Cell. Endocrinology 180:39.
Alternate Names	Activin AB

Citations for Recombinant Human Activin AB Protein (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Activin AB Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

BC Goh et al. (2017), Activin Receptor Type IIA (ACVR2A) Functions Directly in Osteoblasts as a Negative Regulator of Bone Mass J. Biol. Chem., 0(0).
PMID: 28659341
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

E Latres et al. (2017), Activin A more prominently regulates muscle mass in primates than does GDF8 Nat Commun, 8(0):15153.
PMID: 28452368
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ungefroren H et al. (2015), Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta. PLoS ONE, 10(2):e0118097.
PMID: 25707005
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Castonguay R et al. (2011), Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 286(34):30034-46.
PMID: 21737454
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay

Maeshima A et al. (2006), Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct. Dev. Biol., 295(2):473-85.
PMID: 16643884

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Recombinant Human Activin AB Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

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Product Specifications for Recombinant Human Activin AB Protein

Formulation, Preparation and Storage

Carrier Free

1066-AB

Carrier

1066-AB/CF

Carrier Free

Reconstitution Calculator

Background: Activin AB

Citations for Recombinant Human Activin AB Protein (5)

Customer Reviews for Recombinant Human Activin AB Protein (1)

Customer Images

Recombinant Human Activin AB Protein

Product Documents for Recombinant Human Activin AB Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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