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Recombinant Human Active Lyn B Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3206-KS

R&D Systems, part of Bio-Techne
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3206-KS-010

Key Product Details

Source

Sf 9 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Lyn B protein

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Using an N-terminal GST tag

SDS-PAGE

85 kDa

Activity

The specific activity of Lyn B is typically 205-277 nmol/min/mg using a synthetic peptide substrate.

Scientific Data Images for Recombinant Human Active Lyn B Protein, CF

Recombinant Human Active Lyn B Protein SDS-PAGE

Recombinant Human Active Lyn B Protein SDS-PAGE

The approximate molecular weight is 85 kDa and the average purity is 90%.

Formulation, Preparation and Storage

3206-KS
Formulation Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: Lyn B

Lyn B is a tyrosine kinase that is similar to mouse T-lymphocyte-specific tyrosine kinase p56lck, v-yes, v-fgr, and v src and is expressed in a variety of tissues (1). Lyn B is expressed preferentially in B cells and can be co-immunoprecipitated with IgM suggesting that Lyn B is physically associated with membrane-bound IgM, and participates in antigen-mediated signal transduction (2). Cross-linking of membrane-bound IgM with antibody induces a rapid increase in activities of Lyn B and
Lyn-associated phosphatidylinositol 3-kinase.

References

  1. Yamanashi, Y. et al. (1987) Mol. Cell Biol. 7:237.
  2. Yamanashi, Y. et al. (1991) Science 251:192.

Long Name

V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog

Entrez Gene IDs

4067 (Human); 17096 (Mouse); 81515 (Rat)

Gene Symbol

LYN

UniProt

Additional Lyn B Products

Product Documents for Recombinant Human Active Lyn B Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active Lyn B Protein, CF

For research use only

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