What is the activation protocol for this enzyme?

Name: Recombinant Human Active ERK1 Protein, CF
Brand: R&D Systems
SKU: 1879-KS
Price: 453 USD

This enzyme is supplied in its activated form, so no activation step is required.

Recombinant Human Active ERK1 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1879-KS

Citations (2)

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Source:	E. coli
Accession #:	NM_002746
Applications:	Bioactivity

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Product Specifications for Recombinant Human Active ERK1 Protein, CF

Source	E. coli-derived human ERK1 protein
Purity	>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal sequence Analysis	Activated by active MEK1 in vitro
Predicted Molecular Mass	44 kDa
Activity	The specific activity of ERK1 was determined to be 42 nmol/min/mg using a myelin basic protein (MBP) substrate and was equivalent to 382 nmol/min/mg as per radiometric assay.

Scientific Data Examples for Recombinant Human Active ERK1 Protein, CF

	Recombinant Human Active ERK1 Protein SDS-PAGE. The approximate molecular weight is 44 kDa and the purity is > 80%.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1879-KS

Formulation	Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF and 25% glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ERK1

ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.

Item	Value
References	Boulton, T.G. et al. (1991) Biochemistry 30:278.
Long Name	Extracellular Signal-regulated Kinase 1
Entrez Gene IDs	5595 (Human); 26417 (Mouse); 50689 (Rat)
Alternate Names	EC 2.7.11, ERK-1, ERK1, ERK1p44-MAPK, ERT2, Extracellular signal-regulated kinase 1, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, MGC20180, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, P44ERK1, PRKM3, PRKM3EC 2.7.11.24, extracellular signal-related kinase 1, mitogen-activated protein kinase 3, p44-ERK1, p44mapk

Citations for Recombinant Human Active ERK1 Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Active ERK1 Protein, CF include:

Species: Human
Sample Types: Protein
Applications: Bioassay

Y Fan et al. (2022), ERK1/2 inhibits Cullin 3/SPOP-mediated PrLZ ubiquitination and degradation to modulate prostate cancer progression Cell Death and Differentiation, 0(0).
PMID: 35194188
Species: N/A
Sample Types: N/A
Applications: Enzyme Assay

et al. (2011), Phosphatase-coupled universal kinase assay and kinetics for first-order-rate coupling reaction. PLoS ONE, 6(8):e23172.
PMID: 21853082

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FAQs for Recombinant Human Active ERK1 Protein, CF

What is the activation protocol for this enzyme?

This enzyme is supplied in its activated form, so no activation step is required.

Recombinant Human Active ERK1 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human Active ERK1 Protein, CF

Scientific Data Examples for Recombinant Human Active ERK1 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: ERK1

Citations for Recombinant Human Active ERK1 Protein, CF (2)

FAQs for Recombinant Human Active ERK1 Protein, CF

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