Recombinant Human Active Chk1 Protein, CF

Catalog # 1630-KS | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

NM_001274

Source

Sf 9 (baculovirus)

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Chk1 protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Using an N-terminal GST tag

SDS-PAGE

82 kDa

Activity

The specific activity of Chk1 was determined to be 228 nmol/min/mg using a synthetic peptide substrate (KKKVSRSGLYRSPSMPENLNRPR).

Scientific Data Images for Recombinant Human Active Chk1 Protein, CF

Recombinant Human Active Chk1 Protein SDS-PAGE

Recombinant Human Active Chk1 Protein SDS-PAGE.

The approximate molecular weight is 82 kDa and the purity is > 95%.

Formulation, Preparation and Storage

1630-KS
Formulation Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: Chk1

Chk1 is a 56 kDa Serine/Threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle (1). Chk1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by Rad3, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia (2).

References

  1. Walworth, N. et al. (1993) Nature 363:368.
  2. Walworth, N.C. et al. (1996) Science 271:353.

Long Name

Checkpoint Kinase 1

Alternate Names

CHEK1

Entrez Gene IDs

1111 (Human); 12649 (Mouse); 140583 (Rat)

Gene Symbol

CHEK1

UniProt

NM_001274

Product Documents for Recombinant Human Active Chk1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active Chk1 Protein, CF

For research use only

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