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Recombinant Human ACP6 Protein, CF

Bio-Techne includes R&D Systems | Catalog # 7766-AP

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7766-AP-010

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ACP6 protein
Glu33-Glu428, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu33

Predicted Molecular Mass

46 kDa

SDS-PAGE

43-45 kDa, reducing conditions

Activity

Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
The specific activity is >1800 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7766-AP
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ACP6

Lysophosphatidic Acid Phosphatase Type 6 (ACP6) is a membrane-bound protein that belongs to the Histidine Acid Phosphatase Family. ACP6 is expressed in kidney, heart, small intestine, muscle, liver, prostate, testis, ovary, and colon tissues (1, 2). Its function is to hydrolyze lysophosphatidic acid (LPA) to form monoacylglycerol. LPA is one of the simplest of all known phospholipids which has a variety of biological functions including cell proliferation, migration, and survival (3). LPA is found at high levels in serum but also present in saliva, follicular fluid, seminal plasma and malignant effusions and is implicated in many cancers including ovarian, breast and prostate (3-5).

References

  1. Hiroyama, M. and T. Takenawa (1999) J. Biol. Chem. 274:29172.
  2. Takayama I. et al. (2002) Gut 50:790.
  3. Mills, G. and W. Moolenaar (2003) Nature 3:582.
  4. Xu, Y. et al. (1995) Biochem J. 309:933.
  5. Xie, Y. et al. (2002) J. Biol. Chem. 277:32516.

Long Name

Lysophosphatidic Acid Phosphatase 6

Alternate Names

ACPL1, LPAP, PACPL1

Entrez Gene IDs

51205 (Human); 66659 (Mouse); 295305 (Rat)

Gene Symbol

ACP6

UniProt

Additional ACP6 Products

Product Documents for Recombinant Human ACP6 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ACP6 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd. Triton is a registered trademark of Union Carbide Corp.

For research use only

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