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Recombinant Human 5T4 His-tag Avi-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # AVI10290

Biotinylated
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AVI10290-100

Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human 5T4 protein
Human 5T4
(Ser31-Ser355)
Accession # Q13641.1
HHHHHH  Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser31

Predicted Molecular Mass

38 kDa

SDS-PAGE

40-80 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Human 5T4 Antibody (Catalog # MAB49751) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human 5T4 His-tag Avi-tag (Catalog # AVI10290) binds with an ED50 of 10-80 ng/mL.

Scientific Data Images for Recombinant Human 5T4 His-tag Avi-tag Protein, CF

Recombinant Human 5T4 His-tag Avi-tag Protein Binding Activity

Recombinant Human 5T4 His-tag Avi-tag Protein Binding Activity

When Human 5T4 Antibody (Catalog # MAB49751)is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human 5T4 His-tag Avi-tag (Catalog # AVI10290) binds with an ED50 of 10-80 ng/mL.
Recombinant Human 5T4 His-tag Avi-tag Protein SDS-PAGE

Recombinant Human 5T4 His-tag Avi-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human 5T4 His-tag Avi-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-80 kDa.

Formulation, Preparation and Storage

AVI10290
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5T4

5T4, also known as Trophoblast glycoprotein and Wnt-activated inhibitory factor 1 (WAIF1), is a single-pass transmembrane protein which may function as an inhibitor of Wnt/beta-catenin signaling (1). Distinct regions on the 5T4 ectodomain which contains eight leucine-rich repeats are thought to antagonize Wnt signaling (2). 5T4 has limited expression in normal adult tissues and in some solid tumors, while expression is elevated in human carcinomas which have been correlated with poor survival and prognosis (3-5). Human 5T4 is a 420 amino acid (aa) protein that includes a 31 aa signal peptide, a 324 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 44 aa cytoplasmic tail. Within the ECD, human 5T4 shares 78% and 77% aa sequence identity with mouse and rat 5T4, respectively. It has been identified that TIP-2/GIPC, a common interacting protein involved in cancer, interacts with 5T4 through its PDZ domain (6). 5T4 inhibits Wnt/ beta-catenin signaling by binding to LRP6 and inhibit Wnt-induced LRP6 internalization into endocytic vesicles (1). Conversely, 5T4 can activate noncanonical Wnt signaling through DKK1 (1). In addition to LRP6, in-house data indicates 5T4 can also interact with Frizzled-8.

References

  1. Kagermeier-Schenk, B. et al. (2011) Dev. Cell. 21:1129.
  2. Zhao, Y. et al. (2014) Structure. 22:612.
  3. Starzynska, T. et al. (1994) Br. J. Cancer. 69:899.
  4. Naganuma, H. et al. (2002) Anticancer Res. 22:1033.
  5. Harris, J.L. et al. (2018) Int. J. Biochem. Cell Biol. 99:28.
  6. Awan, A. et al. (2002) BBRC 290:1030.

Long Name

Oncofetal Antigen/Trophoblast Glycoprotein

Alternate Names

M6P1, TPBG

Entrez Gene IDs

7162 (Human); 21983 (Mouse); 83684 (Rat); 102132149 (Cynomolgus Monkey)

Gene Symbol

TPBG

UniProt

Additional 5T4 Products

Product Documents for Recombinant Human 5T4 His-tag Avi-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human 5T4 His-tag Avi-tag Protein, CF

For research use only

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