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Recombinant HCoV-229E Nucleocapsid His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10708-CV

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10708-CV-100

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Conjugate

Unconjugated

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived hcov-229e Nucleocapsid protein
Met1-Asn389, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2, determined by protein ID.

Predicted Molecular Mass

44 kDa

SDS-PAGE

48-53 kDa, under reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant HCoV-229E Nucleocapsid His-tag Protein, CF

Recombinant HCoV-229E Nucleocapsid His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant HCoV-229E Nucleocapsid His-tag (Catalog # 10708-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-53 kDa.

Formulation, Preparation and Storage

10708-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nucleocapsid

HCoV-229E belongs to a family of viruses known as coronaviruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N).  HCoV-229E is a member of the alpha-coronavirus family and was discovered in 1966 (1, 2). Other well-known human coronaviruses include three viruses that cause relatively mild respiratory disease: HCoV-NL63, HCoV-HKU1 and HCov-OC43, plus three viruses that caused the Severe Acute Respiratory Syndrome (SARS-CoV), the Middle East Respirator Syndrome (MERS-CoV), and the global pandemic Covid-19 (SARS-CoV2).  While the S, E and M proteins build up the viral envelop, the N protein is involved transcription, replication, and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (3). The CoV-229E N protein is a ~50 kDa protein composed of two independent structural domains connected by a linker region. Both the N-terminal and the linker regions contain RNA binding domains, while the C-terminal region is responsible for the oligomerization of the N protein (4). The CoV‑229E protein shares 45% amino acid sequence identity with CoV-NL63 N protein. the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity. Cross activity of antibodies among different strains should be rigorously tested when designing serological diagnostic kits (5).

References

  1. Hamre, D. and J.J. Procknow (1966) Proc. Soc. Exp. Biol. Med. 121:190.
  2. Van der Hoek, L. et al. (2004) Nat. Med. 10:368.
  3. Chang, C.K. et al. (2006) J.Biomed. Sci.  13:59.
  4. Lo, Y. et al. (2013) FEBS Letters. 587:120.
  5. Chan, K.H. et al. (2005) Clin. Diagn. Lab. Immunol. 12:1317.

Long Name

Nucleocapsid Protein

Alternate Names

N Protein

Entrez Gene IDs

918763 (HCoV-229E); 3200423 (HCoV-HKU1); 2943504 (HCoV-NL63); 39105221 (HCoV-OC43); 14254601 (MERS-CoV); 1489678 (SARS-CoV); 43740575 (SARS-CoV-2)

Gene Symbol

N

UniProt

Additional Nucleocapsid Products

Product Documents for Recombinant HCoV-229E Nucleocapsid His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant HCoV-229E Nucleocapsid His-tag Protein, CF

For research use only

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