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Recombinant H. pylori Fucosyltransferase Protein, CF

Catalog # 11072-GT | R&D Systems, Inc. a Bio-Techne Brand
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11072-GT-020

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived h. pylori Fucosyltransferase protein
Met1-Tyr392, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

46 kDa

SDS-PAGE

39 kDa, under reducing conditions.

Activity

Measured by its ability to transfer fucose from GDP-fucose to N-Acetyllactosamine
The specific activity is >1500 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant H. pylori Fucosyltransferase Protein, CF

Recombinant H. pylori Fucosyltransferase Protein Enzyme Activity Diagram.

Recombinant H. pylori Fucosyltransferase His-tag Protein (Catalog # 11072-GT) Enzyme Activity Diagram.

Fluorescent glycan labeling of asialofetuin (AF) with Cy3-Fuc using rHp. FUT.

Asialofetuin (4 μg) was incubated with 0.2 nmol of GDP-Cy3-Fucose (ES401) with or without rHp. FUT (Catalog # 11072) (1.0 μg) in 25 μL of 25 mM Tris pH 7.5, 10 mM MnCl2 at 37 °C for 30 minutes. Following labeling, half of the AF was deglycosylated with PNGase F N-glycan Releasing Kit (EA006) and resolved in 15% SDS-PAGE. Left side is the TCE image and right side is the Fluorescent image of the same gel.

Recombinant H. pylori Fucosyltransferase Protein SDS-PAGE.

2 μg/lane of Recombinant H. pylori Fucosyltransferase Protein (Catalog # 11072-GT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39 kDa.

Formulation, Preparation and Storage

11072-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Fucosyltransferase

Lewis X (LeX), a fucosylated trisaccharide glycan epitope (Gal beta1,4 [Fuc alpha1,3]GlcNAc beta) also known as CD15, and sialylated Lewis X (sLeX) are distributed throughout eukaryotes and are determinants of many functional glycoconjugates that play central roles in numerous physiological and pathological processes (1, 2). Lex-bearing glycans are also found in the infectious bacterium Helicobacter pylori to mask the bacterium from the host immune surveillance (3). H. pylori is the pathogen that causes peptic ulcers that can further lead to stomach cancer and gastritis. H. pylori fucosyltransferase is responsible for generating the LeX glycans, therefore is potentially a drug target for curing peptic ulcers, gastritis and stomach cancer. In molecular terms, H. pylori fucosyltransferase is an  alpha1,3 fucosyltransferase that shares function with human FUT4, FUT5, FUT6, and FUT9 (4). Remarkably, H. pylori fucosyltransferase can transfer IgG antibody to the glycocalyx on the surfaces of live cells when the antibody is conjugated to the enzyme's natural donor substrate GDP-Fucose (5). The activity of this enzyme has been measured with a phosphatase coupled method (6).

References

  1. Gooi, H.C. et al. (1981) Nature 292:156.
  2. Phillips, M.L. et al. (1990) Science 250:1130.
  3. Moran, A.P. et al. (1996) FEMS Immunol Med Microbiol 16:105.
  4. de Vries, T. et al. (2001). Glycobiology 11:119R.
  5. Li, J. et al. (2018) ACS Cent. Sci. 4:1633.
  6. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

alpha-(1,3) Fucosyltransferase FucT

Alternate Names

FucT

UniProt

Additional Fucosyltransferase Products

Product Documents for Recombinant H. pylori Fucosyltransferase Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant H. pylori Fucosyltransferase Protein, CF

For research use only

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