Recombinant Feline IFN-gamma Protein

Name: Recombinant Feline IFN-gamma Protein
Brand: R&D Systems
SKU: 764-FG

Catalog # 764-FG | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

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Key Product Details

Source:	E. coli
Accession #:	P46402
Applications:	Bioactivity

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Technical Data
Citations (1) / Reviews

Product Specifications for Recombinant Feline IFN-gamma Protein

Source	E. coli-derived feline IFN-gamma protein Gln24-Lys167, with an N-terminal Met
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	17.1 kDa
SDS-PAGE	17.1 kDa
Activity	Measured in an anti-viral assay using CRFK feline kidney epithelial cells infected with vesicular stomatitis virus (VSV). The ED₅₀ for this effect is 0.15‑0.9 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

764-FG

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

764-FG/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature feline IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3, 4). It shares 88% amino acid sequence identity with canine IFN-gamma, 72% - 78% with bovine, equine, and porcine IFN-gamma, and 40% - 62% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (5, 6). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (7). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (7, 8). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (9, 10). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (8).

Item	Value
References	Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. Pestka, S. et al. (2004) Immunol. Rev. 202:8. Argyle, D.J. et al. (1995) DNA Seq. 5:169. Schijns, V.E. et al. (1995) Immunogenetics 42:440. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names	IFG, IFI, IFN-gamma, IFNG, IFNgamma, Immune interferon, interferon gamma, interferon, gamma

Citations for Recombinant Feline IFN-gamma Protein (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Feline IFN-gamma Protein include:

Species: Feline
Sample Types: Whole Cells
Applications: Bioassay

RM Troyer et al. (2019-09-07), Expression of APOBEC3 Lentiviral Restriction Factors in Cats Viruses, 2019-011(9).
PMID: 31500260

Recombinant Feline IFN-gamma Protein

Catalog # 764-FG | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Feline IFN-gamma Protein

Formulation, Preparation and Storage

Carrier Free

764-FG

Carrier

764-FG/CF

Carrier Free

Reconstitution Calculator

Background: IFN-gamma

Citations for Recombinant Feline IFN-gamma Protein (1)

Product Documents for Recombinant Feline IFN-gamma Protein

Product Datasheets

COA

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