Recombinant Equine PDGF-BB Protein

Name: Recombinant Equine PDGF-BB Protein
Brand: R&D Systems
SKU: 8585-BB

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 8585-BB

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Key Product Details

Source:	E. coli
Accession #:	XP_005606703
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Equine PDGF-BB Protein

Product Specifications for Recombinant Equine PDGF-BB Protein

Source	E. coli-derived equine PDGF-BB protein Ser51-Thr159
Purity	>95%, by SDS-PAGE with silver staining
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser51
Predicted Molecular Mass	12 kDa
SDS-PAGE	12 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 5-30 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

8585-BB

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8585-BB/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF-BB

Platelet-Derived Growth Factor (PDGF)-BB is synthesized as a prepro-precurser that contains a signal peptide, an N-terminal prodomain, a mature region, and a
C-terminal prodomain (1-4). The proprecursor is initially dimerized and then intracellulary processed twice. The N-terminal prodomain is cleaved first, followed by cleavage of the C-terminal prodomain prior to the formation of a disulfide-linked homodimer. Mature equine PDGF-B shares 95%, 88%, 89%, 83%, 79%, and 79% amino acid sequence identity with human, mouse, rat, sheep, cow, and goat PDGF-B, respectively. PDGF-BB is expressed by hepatocytes and nonresorbing osteoclasts, generating osteoblasts and bone formation (4, 5). It is also produced by platelets, macrophages, and mast cells. At sites of injury, it promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new extracellular matrix, and IGF-I-mediated re-epithelialization (6, 7). The traditional receptor for PDGF is either a homodimer or heterodimer created from two type I transmembrane RTKs, PDGF R alpha and PDGF R beta (8, 9). PDGF-BB has been shown to bind the alpha alpha homodimer, alpha beta heterodimer, and the beta beta homodimer in vitro, and act through the beta beta homodimer in vivo (8, 10). Equine PDGF-BB has been shown to stimulate contraction of fibroblast-populated collagen matrix, a model of equine wound contraction, in a Rho kinase- and p38-dependent manner (12). PDGF-BB is present in platelet-rich plasma (PRP), which has been used to treat soft tissue injuries in horses (13, 14). The levels of equine PDGF-BB in PRP is dependent on breed, gender, and age (15).

Item	Value
References	Rao, C.D. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2392. Kaetzel, D.M. et al. (1996) Biochim. Biophys. Acta. 1298:250. Ostman, A. et al. (1992) J. Cell Biol. 118:509. Siegfried, G. et al. (2005) Oncogene 24:6925. Kreja, L. et al. (2010) J. Cell. Biochem. 109:347. van Steensel, L. et al. (2012) J. Clin. Endocrinol. Metab. 97:E400. Barrientos, S. et al. (2008) Wound Repair Regen. 16:585. Andrae, J. et al. (2008) Genes Dev. 22:1276. Heldin, C.H. and B. Westermark (1999) Physiol. Rev. 79:1283. Li, X. and U. Eriksson (2003) Cytokine Growth Factor Rev. 14:91. Watts, E.J. and M.T. Rose (2010) Domest. Anim. Endocrinol. 38:253. Boswell, S.G. et al. (2014) Am. J. Sports Med. 42:42. Halper, J. (2014) Adv. Exp. Med. Biol. 802:59. Giraldo, C.E. et al. (2013) BMC Vet. Res. 9:29.
Long Name	Platelet-derived Growth Factor BB
Entrez Gene IDs	5155 (Human); 24628 (Rat)
Alternate Names	PDGF-BB, PDGFBB

Citations for Recombinant Equine PDGF-BB Protein

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Recombinant Equine PDGF-BB Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Equine PDGF-BB Protein

Formulation, Preparation and Storage

Carrier Free

8585-BB

Carrier

8585-BB/CF

Carrier Free

Reconstitution Calculator

Background: PDGF-BB

Citations for Recombinant Equine PDGF-BB Protein

Product Documents for Recombinant Equine PDGF-BB Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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