>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Ile33 & Glu502
Predicted Molecular Mass
63-75 kDa, under non-reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Viral EBOV GP (mucin domain deleted, Catalog # 10585-EB) is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human CLEC10A/CD301 (Catalog # 4888-CL) binds with an ED50 of 1-10 ng/mL.
Recombinant EBOV GP (Mucin Domain Deleted) Protein, CF Scientific Data Examples
Recombinant EBOV GP (mucin domain deleted) Protein Binding Activity.
When Recombinant Viral EBOV GP (Mucin Domain Deleted) Protein (Catalog # 10585-EB) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CLEC10A/CD301 Protein (4888-CL) binds with an ED50 of 1-10 ng/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EBOV GP
The GP glycoprotein encoded by the genome of Ebola family viruses is a critical molecule for the pathogenicity of Ebolavirus hemorrhagic viruses (1, 2). It is processed into distinct forms for virus capsule or cell surface presentation or release from virus infected cells. The GP precursor protein is cleaved by furin at a multibasic site to yield a 140 kDa N-terminal fragment (GP1) and a 26 kDa C-terminal fragment (GP2) which remain disulfide linked (3). GP1 is entirely extracellular while GP2 is a transmembrane protein (4). Heterodimers of GP1-GP2 can further associate into trimers (5). GP expressed on virus infected cells can be shed by TACE mediated cleavage, liberating a disulfide linked complex of soluble GP1 and truncated GP2 (4-6). GP binds to multiple C-type lectins on target cell surfaces, including CLEC10A/MGL, DC-SIGN, and DC-SIGNR (7-9). Following internalization, GP1 is cleaved by Cathepsin B and Cathepsin L and then interacts with Niemann-Pick C1 (NPC1) in the endosomal membrane (10-12).
Yang, Z.-Y. et al. (2000) Nat. Med. 6:886.
de La Vega, M.-A. et al. (2015) Viral Immunol. 28:3.
Volchkov, V.E. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5762.
Volchkov, V.E. et al. (1998) Virology 245:110.
Sanchez, A. et al. (1998) J. Virol. 72:6442.
Dolnik, O. et al. (2004) EMBO J. 23:2175.
Takada, A. et al. (2004) J. Virol. 78:2943.
Alvarez, C.P. et al. (2002) J. Virol. 76:6841.
Simmons, G. et al. (2003) Virology 305:115.
Schornberg, K. et al. (2006) J. Virol. 80:4174.
Chandran, K. et al. (2005) Science 308:1643.
Cote, M. et al. (2011) Nature 477:344.
Ebola Virus Glycoprotein
Entrez Gene IDs
EBOV GP, Spike glycoprotein, bovGP, second secreted glycoprotein, small secreted glycoprotein
Citations for Recombinant EBOV GP (Mucin Domain Deleted) Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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