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Recombinant E. coli Ecotin Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1328-PI

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1328-PI-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

E. coli-derived e. coli Ecotin protein
Ala21-Arg162, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21

Predicted Molecular Mass

17 kDa

SDS-PAGE

18 kDa, reducing conditions

Activity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <1.0 nM, as measured under the described conditions.

Formulation, Preparation and Storage

1328-PI
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile 25 mM Tris and 350 mM NaCl, pH 8.5.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ecotin

Ecotin is a potent and general inhibitor of serine proteases with diverse substrate specificities (1). It strongly inhibits trypsin, chymotrypsin, elastase, factor Xa, plasma kallikrein, u-plasminogen activator, granzyme B, and factor XIIa. Immobilized Ecotin has been used to affinity-purify recombinant trypsinogen, indicating that it may also be used to purify additional serine protease zymogens (2). Compared to other serine protease inhibitors such as members of the serpin family, the reactive site of Ecotin is Met104 (P1) (3). Ecotin is synthesized as a 162 amino acid precursor with a 20 amino acid signal peptide necessary to direct it to the periplasmic space. The mature protein (residues 21‑162) is expressed and purified.

References

  1. McGrath, M.E. et al. (1995) Protein Sci. 4:141.
  2. Lengyel, Z. et al. (1998) Protein Expre. Purif. 12:291.
  3. McGrath, M.E. et al. (1991) J. Biol. Chem. 28:6620.

UniProt

Additional Ecotin Products

Product Documents for Recombinant E. coli Ecotin Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant E. coli Ecotin Protein, CF

For research use only

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Reconstitution Calculator

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