Recombinant Cynomolgus TNF RII/TNFRSF1B Fc Protein, CF

Catalog # 10377-TR | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

XP_005544817.1

Source

CHO

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey TNF RII/TNFRSF1B protein
Cynomolgus Monkey TNF RII/TNFRSF1B
(Leu23-Asp257)
Accession # XP_005544817.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

36 kDa

SDS-PAGE

68-80 kDa, under reducing conditions

Activity

Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.
The ED50 for this effect is 0.2-1.2 ng/mL in the presence of 0.25 ng/mL of Recombinant Human TNF-alpha (Catalog # 210-TA).

Scientific Data Images for Recombinant Cynomolgus TNF RII/TNFRSF1B Fc Protein, CF

Recombinant Cynomolgus TNF RII/TNFRSF1B Fc Protein Bioactivity

Recombinant Cynomolgus TNF RII/TNFRSF1B Fc Protein Bioactivity

Recombinant Cynomolgus Monkey TNF RII/TNFRSF1B Fc Chimera (Catalog # 10377-TR) inhibits Recombinant Human TNF-a (Catalog # 210-TA) mediated cytotoxicity in the L-929 mouse fibroblast cells. The ED50 for this effect is 0.2-1.2 ng/mL in the presence of 0.25 ng/mL of Recombinant Human TNF-alpha.
Recombinant Cynomolgus TNF RII/TNFRSF1B Fc Protein SDS-PAGE

Recombinant Cynomolgus TNF RII/TNFRSF1B Fc Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey TNF RII/TNFRSF1B Fc Chimera (Catalog # 10377-TR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-80 kDa and 136-160 kDa, respectively.

Formulation, Preparation and Storage

10377-TR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RII/TNFRSF1B

Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily (1-4). The TNF receptor superfamily is comprised of structurally related receptors that bind to TNF-related ligands and regulate numerous processes such as immune cell activation and apoptosis. Receptors in this superfamily are characterized by the presence of a cysteine-rich region in their extracellular domain (ECD) (1-3, 5). Cynomolgus monkey TNF RII shares 97% sequence identity in the ECD with its human homolog. Several receptors in the TNF superfamily also contain intracellular death domains (DDs) that recruit caspase-interacting proteins to initiate apoptosis upon ligand binding. Those receptors that lack DDs, like TNF RII, bind TNF Receptor-associated Factors, which transduce signals generated by activation of these receptors (6, 7). TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes, neurons, oligodendrocytes, cardiac myocytes, and thymocytes (6, 8, 9). TNF RII activation primarily initiates pro-inflammatory and pro-survival responses via NF kappa B-dependent signaling pathways (6, 7). However, under certain conditions, TNF RII signaling can induce apoptosis (8). Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors (10).

References

  1. Dembic, Z. et al. (1990) Cytokine 2:231.
  2. Kohno, T. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8331.
  3. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.
  4. Loetscher, H. et al. (1990) J. Biol. Chem. 265:20131.
  5. Beltinger, C.P. et al. (1996) Genomics 35:94.
  6. Faustman, D. and M. Davis (2010) Nat. Rev. Drug Discov. 9:482.
  7. Ihnatko, R. and M. Kubeš (2007) Gen. Physiol. Biophys. 26:159.
  8. Mason, A.T. et al. (1995) J. Leukoc. Biol. 58:249.
  9. Speeckaert, M.M. et al. (2012) Am. J. Nephrol. 36:261.
  10. Sennikov, S.V. et al. (2014) Mediators Inflamm. 2014:745909.

Long Name

Tumor Necrosis Factor Receptor II

Alternate Names

CD120b, TNFRII, TNFRSF1B

Entrez Gene IDs

7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)

Gene Symbol

TNFRSF1B

UniProt

XP_005544817.1

Product Documents for Recombinant Cynomolgus TNF RII/TNFRSF1B Fc Protein, CF

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Product Specific Notices for Recombinant Cynomolgus TNF RII/TNFRSF1B Fc Protein, CF

For research use only

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