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Recombinant Cynomolgus Siglec-9 Fc Chimera Protein, CF

Catalog # 11242-SL | R&D Systems, Inc. a Bio-Techne Brand
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11242-SL-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey Siglec-9 protein
Cynomolgus Siglec-9
Gln20-Gly350 (Trp165Arg, Glu316Asp)
Accession # XP_005590140.2
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln20; deduced from Arg21 after deblocking

Predicted Molecular Mass

63 kDa

SDS-PAGE

81-91 kDa, under reducing conditions.

Activity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells.
The ED50 for this effect is 40.0-480 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Siglec-9 Fc Chimera Protein, CF

Recombinant Cynomolgus Siglec-9 Fc Chimera Protein Bioactivity.

Recombinant Cynomolgus Monkey Siglec‑9 Fc Chimera (Catalog # 11242-SL) supports the adhesion of human red blood cells. The ED50 for this effect is 40.0-480 ng/mL.

Recombinant Cynomolgus Siglec-9 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Siglec-9 Fc Chimera Protein (Catalog # 11242-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 81-91 kDa and 160-180 kDa, respectively.

Formulation, Preparation and Storage

11242-SL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-9

Siglecs(1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec-5 to -11 (1 - 4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

The cDNA of cynomolgus Siglec-9 encodes a 464 amino acid (aa) polypeptide. Based on the similarity as human Siglec-9,  cynomolgus Siglec-9 is predicted to contain a signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5, 6). In peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T cells (5). It binds equally well to both 2,3- and 2,6-linked sialic acid (5, 6). Siglec-9 is closely related to Siglec-7, and they share ~80% amino acid sequence identity. The gene encoding siglec-9 was mapped to chromosome 19q13.4.

References

  1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
  2. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  3. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
  5. Zhang, J.Q. et al. (2000) J. Biol. Chem. 275:22121.
  6. Angata, T. et al. (2000) J. Biol. Chem. 275:22127.

Long Name

Sialic Acid Binding Ig-like Lectin 9

Alternate Names

CD329, OBBP-Like, Siglec9

Entrez Gene IDs

27180 (Human); 102118310 (Cynomolgus Monkey)

Gene Symbol

SIGLEC9

UniProt

Additional Siglec-9 Products

Product Documents for Recombinant Cynomolgus Siglec-9 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Siglec-9 Fc Chimera Protein, CF

For research use only

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