Recombinant Cynomolgus/Rhesus Macaque CD38 Protein, CF

Name: Recombinant Cynomolgus/Rhesus Macaque CD38 Protein, CF
Brand: R&D Systems
SKU: 9834-AC
Price: 570 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9834-AC

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Source:	HEK293
Accession #:	Q5VAN0
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Cynomolgus/Rhesus Macaque CD38 Protein, CF

Product Specifications for Recombinant Cynomolgus/Rhesus Macaque CD38 Protein, CF

Source	Human embryonic kidney cell, HEK293-derived cynomolgus monkey CD38 protein Leu44-Ile301, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu44
Predicted Molecular Mass	31 kDa
SDS-PAGE	36-44 kDa, reducing conditions
Activity	Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD⁺) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260. The specific activity is >2,900 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9834-AC

Formulation	Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: CD38

CD38, also known as ADP‑ribosyl cyclase, converts NAD(P)⁺ into three separate products with calcium mobilizing ability: cyclic ADP-ribose, NAADP⁺, and ADP‑ribose (1). CD38 is a Type II transmembrane glycoprotein composed of an intracellular domain, a single transmembrane helix domain, and a large extracellular domain that contains the catalytic site (2). Within the extracellular domain, cynoCD38 shares 93% amino acid sequence identity with human CD38. CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (3, 4). Through its production of cyclic ADP‑ribose, CD38 modulates calcium‑mediated signal transduction in many types of cells (5, 6). CD38 is also reported to bind as a receptor to trigger signaling cascades (7, 8). Through both mechanisms, CD38 influences proliferation and trafficking (8, 9). CD38 is used as a marker for poor prognosis in chronic lymphocytic leukemia and multiple myeloma and is an attractive cancer immunotherapy drug target (8-11).

Item	Value
References	Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249. Liu, Q. et al. (2005) Structure. 13:1331. Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811. Sun, L. et al. (1999) J. Cell Biol. 146:1161. Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209. Kato, I. et al. (1995) J. Biol. Chem. 270:30045. Deaglio, S. (2000) Chem. Immunol. 75:99. Chillemi, A. et al. (2013) Mol. Med. 19:99. Vaisitti, T. et al. (2015) Leukemia. 29:356. Atanackovic, D. et al. (2016) Oncoimmunology. 5:e1217374. Tai, Y-T. et al. (2017) Oncotarget. 8:112166.
Long Name	Cluster of Differentiation 38
Entrez Gene IDs	952 (Human); 12494 (Mouse); 25668 (Rat); 102126394 (Cynomolgus Monkey)
Alternate Names	2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase (EC:2.4.99.20), 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl Cyclase, ADP-ribosyl cyclase 1, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, ADPRC 1, ADPRC1, CD38, CD38 antigen, CD38 antigen (p45), CD38 molecule, CD_antigen: CD38, Cyclic ADP-ribose Hydrolase, Cyclic ADP-ribose hydrolase 1, EC 2.4.99.20, EC 3.2.2.6, EC:3.2.2.6, NAD(+) nucleosidase, T10, cADPr hydrolase 1, cluster of differentiation 38

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Recombinant Cynomolgus/Rhesus Macaque CD38 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Cynomolgus/Rhesus Macaque CD38 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: CD38

Citations for Recombinant Cynomolgus/Rhesus Macaque CD38 Protein, CF

Product Documents for Recombinant Cynomolgus/Rhesus Macaque CD38 Protein, CF

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