Recombinant Cynomolgus/Rhesus Hepassocin/FGL1 Fc Protein, CFNew

Name: Recombinant Cynomolgus/Rhesus Hepassocin/FGL1 Fc Protein, CF
Brand: R&D Systems
SKU: 11176-HE

Catalog # 11176-HE

Product Datasheet / COA / SDS

Fc Chimera

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Key Product Details

Source:	CHO
Accession #:	XP_005562748.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Cynomolgus/Rhesus Hepassocin/FGL1 Fc Protein, CF

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived Hepassocin/FGL1 protein

MD	Human IgG₁ Fc (Pro100-Lys330)	IEGR	Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1 (Ala63-Ile348) Accession # XP_005562748.1
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Met (of Fc)

Predicted Molecular Mass

60 kDa

SDS-PAGE

58-65 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human LAG-3 Fc Chimera Protein (Catalog # 2319-L3) is immobilized at 2.50 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1 Fc Chimera (Catalog # 11176-HE) binds with an ED₅₀ of 0.500-4.00 µg/mL.

Recombinant Cynomolgus/Rhesus Hepassocin/FGL1 Fc Protein, CF Scientific Data Examples

	Recombinant Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1 Fc Chimera Protein Binding Activity. Measured by its binding ability in a functional ELISA. When Recombinant Human LAG-3 Fc Chimera Protein (2319-L3) is immobilized at 2.50 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1 Fc Chimera Protein (Catalog # 11176-HE) binds with an ED₅₀ of 0.500-4.00 µg/mL.
	Recombinant Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1 Fc Chimera Protein SDS-PAGE. 2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1 Fc Chimera Protein (Catalog # 11176-HE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-65 kDa and 120-130 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11176-HE

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and Glycerol with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Hepassocin/FGL1

Hepassocin, also known as Hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and Fibrinogen-Like Protein 1 (FGL1) (1), is a liver-specific secreted protein belonging to the fibrinogen superfamily whose members share a fibrinogen domain at their C-termini (2). Human Hepassocin/FGL1 is a secreted homodimer consisting of 312 amino acids (aa) with a 22 aa signal sequence and a 290 aa mature protein (3). Cynomolgus monkey shares 100% and 96.5% amino acid identity with rhesus macaque and human Hepassocin/FGL1, respectively. Hepassocin/FGL1 binds LAG-3 through its fibrinogen-like domain independently of MHC class II (4). Hepassocin/FGL1 inhibits antigen-specific T cell activation, with its elevated presence in plasma of cancer patients indicating poor prognoses (4). Other than this role in cancer progression, Hepassocin/FGL1 also has restorative function for liver cells. It is upregulated during liver regeneration following partial hepatectomy (5), and stimulates proliferation of hepatocytes in vivo and improves prognoses with fulminant hepatic failure in rats (6). Its expression is regulated in Hep G2 cells by interleukin-6 (IL-6) and is found in the serum in both bound and unbound states as an acute phase reactant (7).

Item	Value
References	Yamamoto, T. et al. (1993) Biochem. Biophys. Res. Commun. 2:681. Zhang, S.M. et al. (2008) Innate Immun. 14:175. Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45. Wang, J. et al. (2019) Cell. 176:334. Yu, HT. et al. (2009) J. Biol. Chem. 284:13335. Li, C.Y. et al. (2010) Gut. 59:817. Liu, Z. and C. Ukomadu. (2008) Biochem. Briophys. Res. Commun. 365:729.
Long Name	Fibrinogen Like 1
Entrez Gene IDs	2267 (Human); 246186 (Rat); 102118020 (Cynomolgus Monkey)
Alternate Names	FGL1, FREP1, Fibrinogen-like-protein 1, HFREP-1, HFREP1MGC12455, HP-041, Hepassocin, Hepatocyte-derived fibrinogen-related protein 1, LFIRE-1, LFIRE1, Liver fibrinogen-related protein 1, fibrinogen-like 1, fibrinogen-like protein 1, hepatocellular carcinoma-related sequence

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