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Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF

Catalog # 10509-GP | R&D Systems, Inc. a Bio-Techne Brand
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10509-GP-050

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived Glypican 3 protein
Gln25-Asn554, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln25 (blocked) & Ser359

Predicted Molecular Mass

38 kDa (alpha chain), 23 kDa (beta chain), 61 kDa (full length)

SDS-PAGE

65-90 kDa, under non-reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human FGF basic/FGF2 (146aa) (Catalog # 233-FB/CF) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Glypican 3 His-tag (Catalog # 10509-GP) binds with an ED50 of 0.075-0.45 μg/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF

Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein Binding Activity

Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein Binding Activity

When Recombinant Human FGF basic/FGF2 (146 aa) Protein (233-FB/CF) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Glypican 3 His-tag Protein (10509-GP) binds with an ED50  of 0.075-0.45 μg/mL.

Formulation, Preparation and Storage

10509-GP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 3

Glypican 3 (GPC3), also known as GTR2-2, intestinal protein OCI-5 and MXR7, is a member of the heparan sulfate (HS) proteoglycan family (1).  In mammals, six glypican family members have been identified, all sharing a conserved core protein that is linked to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. GPC3 can be cleaved by furin to produce two subunits that are linked by disulfide bonds: a 40 kDa N-terminal alpha subunit that can be secreted into the blood and a 30 kDa membrane-bound C-terminal beta subunit containing two HS glycan chains (1-3). Several isoforms of GPC3 due to alternative splicing have been reported (1). Within the mature extracellular domain, cynomolgus/rhesus GPC3 shares 99% amino acid sequence identity with human GPC3.  GPC3 is an important biomarker present in the serum of hepatocellular carcinoma patients, which distinguishes benign from cancerous nodules (4). Glypican 3 is over-expressed in hepatocellular carcinomas and binding of GPC3 to CD81 promotes development of carcinomas by activation of Hippo pathways in hepatocytes (5).  Our Avi-tag Biotinylated Recombinant  Glypican 3  features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Ho, M. and Kim, H. (2012) Eur. J. Cancer. 47:333.
  2. Haruyama, Y. and Kataoka, H. (2016) World J. Gastroenterol. 22:275.
  3. Shimizu, Y. et al. (2019) Front Oncol. 9:248.
  4. Ge, S. et al. (2018) Filmus, Int J Clin Exp Pathol. 11(12):5774.
  5. Xue, Y. et al. (2018) Am J Pathol. 188(6):1469.

Alternate Names

GPC3

Entrez Gene IDs

2719 (Human); 14734 (Mouse); 25236 (Rat); 102137748 (Cynomolgus Monkey)

Gene Symbol

GPC3

UniProt

Product Documents for Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF

For research use only

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