Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

Name: Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF
Brand: R&D Systems
SKU: 10437-ZN

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10437-ZN

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Key Product Details

Source:	CHO
Accession #:	XP_005579379.1
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

Product Specifications for Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey PSMA/FOLH1/NAALADase I protein Lys44-Ala750 with an N-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	His
Predicted Molecular Mass	80 kDa
SDS-PAGE	94-105 kDa, reducing conditions
Activity	Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >200 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

	Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein SDS-PAGE 2 μg/lane of Recombinant Cynomolgus Monkey PSMA/FOLH1/NAALADase I His-tag (Catalog # 10437-ZN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at ~100 kDa under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10437-ZN

Formulation	Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PSMA/FOLH1/NAALADase I

Prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1,2). PMSA has a short cytosolic N-terminal domain, a single membrane-spanning segment, and an extracellular region that is composed of a protease domain, apical domain, and C-terminal domain (3). The extracellular domains all contribute to substrate recognition. The protein forms an active homodimer reliant on interactions between amino-acid side chains and glycosylation (3,4). PSMA is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, and N-acetylated-alpha-linked acidic dipeptidase-1 (NAALADase1). PSMA activity plays a role in tumor angiogenesis making it not only a tumor marker, but a therapeutic target in cancers including prostate cancer (5). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu (NAAG) to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity such as stroke, schizophrenia, Alzheimer's, and amyotrophic lateral sclerosis (6,7,8). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (8). Upregulation of PSMA is present in inflammatory bowel disease, Crohn's disease, and ulcerative colitis where pharmacological inhibition has shown amelioration of clinical symptoms pertaining to these diseases in mice (5).

Item	Value
References	Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81. Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA. 93:749. Mesters, J.R. et al. (2006) EMBO J. 25:1375. Shulke, N. et al. (2003) Proc. Natl. Acad. Sci. USA. 100:12590. Vornov, J.J. et al. (2019) Neurochem. Res. [Epub ahead of print]. Jackson, P.F. and Slusher, B.S. (2001) Curr. Med. Chem. 8:949. Neale, J.J. and T. Yamamoto. (2020) Prog. Neurobiol. 184:101722. Heston, W.D. (1997) Urology 49:104.
Long Name	Prostate-specific Membrane Antigen
Entrez Gene IDs	2346 (Human); 53320 (Mouse); 85309 (Rat); 101866140 (Cynomolgus Monkey)
Alternate Names	Cell growth-inhibiting gene 27 protein, EC 3.4.17.21, FGCP, FOLH1, FOLHNAALADase I, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, GCP2, GCPII, GCPIImGCP, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated alpha-linked acidic dipeptidase 1, NAALAD1, NAALAD1N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, NAALAdase, PSMA, PSMAFGCP, PSMPteroylpoly-gamma-glutamate carboxypeptidase, Prostate-specific membrane antigen, cell growth-inhibiting protein 27, folate hydrolase (prostate-specific membrane antigen) 1, glutamate carboxylase II, glutamate carboxypeptidase 2, mopsm, prostate specific membrane antigen variant F

Citations for Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Product Specifications for Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

Scientific Data Examples for Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: PSMA/FOLH1/NAALADase I

Citations for Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

Product Documents for Recombinant Cynomolgus PSMA/FOLH1/NAALADase I Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected