Human embryonic kidney cell, HEK293-derived cynomolgus monkey VSIG4 protein Arg25-Pro288, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
35-45 kDa, under reducing conditions
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 0.30-3.00 μg/mL
Recombinant Cynomolgus Monkey VSIG4 His-tag Protein, CF Scientific Data Examples
Recombinant Cynomolgus Monkey VSIG4 His-tag Protein Bioactivity.
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 0.30-3.00 μg/mL.
Recombinant Cynomolgus Monkey VSIG4 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Cynomolgus Monkey VSIG4 His-tag (Catalog # 10807-VS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-45 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 200 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
VSIG4 (V-set and immunoglobulin domain containing 4), also known as CRIg and Z39IG, is a 45 kDa, type I transmembrane protein of the B7 family within the Ig superfamily that is expressed only in tissue-resident macrophages (1-4). The cynomolgus VSIG4 cDNA encodes 404 amino acids (aa) including a 24 aa signal sequence, a 264 aa extracellular domain (ECD) containing a V-type and a C2-type Ig domain, a 23 aa transmembrane domain and a 93 aa cytoplasmic domain (5). The cynomolgus VSIG4 ECD shares 94% aa identity with human VSIG4 ECD. VSIG4 is specifically expressed on macrophages in the thymic medulla, peritoneum, alveoli, synovia, adipose and heart, liver Kupffer cells, placental Hofbauer cells, and atherosclerotic foam cells (1-4, 6-9). It is absent on infiltrating macrophages (8). VSIG4 is a complement receptor that binds C3b and iC3b fragments, internalizes them to recycling endosomes, and is recycled to the cell surface (4, 6). It contributes significantly to innate immunity by binding and phagocytosis of complement-opsonized invading pathogens (4, 8, 10). Binding of either native or recombinant soluble VSIG4 to C3b inhibits complement amplification through the alternative, but not classical, pathway (10, 11). VSIG4 is also a negative regulator of mouse and human T cell activation (2). Although VSIG4 engagement may activate NF kappa B and thus be pro-inflammatory in some cases, many of its activities are important in resolving, rather than initiating, inflammation (1, 2, 7, 10, 11).
He, J.Q. et al. (2008) Mol. Immunol. 4041.
Vogt, L. et al. (2006) J. Clin. Invest. 116:2817.
Langnaese, K. et al. (2000) Biochim. Biophys. Acta 1492:522.
Helmy, K. et al. (2006) Cell 124:915.
Entrez protein Accession # A0A2K5TW73, XP_005593850.
Tanaka, M. et al. (2008) Clin. Exp. Immunol. 154:38.
CRIg, Ig superfamily protein, Protein Z39Ig, V-set and immunoglobulin domain containing 4, V-set and immunoglobulin domain-containing protein 4, VSIG4, Z39IG, Z39IGcomplement receptor of the immunoglobulin superfamily
Citations for Recombinant Cynomolgus Monkey VSIG4 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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