>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
38-50 kDa, under reducing conditions
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect 1-10 μg/mL.
Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein, CF Scientific Data Examples
Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein Bioactivity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 1-10 μg/mL.
Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein SDS-PAGE
2 μg/lane of Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein (Catalog # 10530-L6) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-50 kDa and 76-100 kDa, respectively.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 200 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lymphocyte antigen 6E (Ly6E), also known as Stem cell antigen 2 (SCA-2) or Thymic shared antigen-1 (TSA-1), is a member of the lymphostromal cell membrane Ly6 protein superrfamily (1, 2). There are at least twenty different human Ly6 proteins that have been identified, and they are either secreted or GPI-anchor membrane-bound proteins (3). Cynomolgus Ly6E is synthesized as a precursor molecule that includes a signal peptide, a Ly6E chain, and a propeptide. Within the mature, main chain region, cynomolgus Ly6E shares 94% amino acid sequence identity with human Ly6E. Ly6E is involved in cell signalling transduction, cell adhesion, immune regulation and drug resistance, and is over-expressed in human malignancies, including head and neck squamous cell carcinomas and lung, and oesophageal cancers (4-7). Moreover, Ly6E over-expresses in gastric cancer cells, and is important for cell survival, proliferation and migration, and could be a novel oncogenic protein for efficient diagnosis and treatment of gastric cancer (2). LY6E has recently been shown to promote viral entry of some flaviviruses, including West Nile virus, Dengue virus, and Zika virus (8). Our in-house data indicate that Ly6E inhibits the human T cell activation, including IL-2, IFN-g secretions.
Gumley, T.P. et al. (1995) Immunol. Cell Biol. 73:277.
Lv, Y. et al. (2018) Cell. Physiol. Biochem. 45:1219.
Kong, H.K. and J.H. Park. (2012) BMB Rep. 45:595.
Luo, L. et al. (2016) Oncotarget 7:11165.
Yeom, C.J. et al. (2016) Oncotarget 7:65837.
AlHossiny, M. et al. (2016) Cancer Res. 76:3376.
Asundi, J. et al. (2015) Clin Cancer Res. 21:3252.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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