Recombinant Cynomolgus Monkey IFN-alpha/beta R1 Protein, CF

Name: Recombinant Cynomolgus Monkey IFN-alpha/beta R1 Protein, CF
Brand: R&D Systems
SKU: 10674-AB

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10674-AB

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His-tag

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Key Product Details

Source:	HEK293
Accession #:	EHH61907.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Cynomolgus Monkey IFN-alpha/beta R1 Protein, CF

Product Specifications for Recombinant Cynomolgus Monkey IFN-alpha/beta R1 Protein, CF

Source	Human embryonic kidney cell, HEK293-derived cynomolgus monkey IFN-alpha/beta R1 protein Ala23-Ile439, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala23 & Gly26
Predicted Molecular Mass	48 kDa
SDS-PAGE	80-100 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinan Cynomolgus Monkey IFN‑ alpha / beta R1 His-tag protein is immobilized at 10 µg/mL (100 µL/well), Recombinant Human IFN-A2 binds with an ED₅₀of 1-6 μg/mL.

Scientific Data Examples for Recombinant Cynomolgus Monkey IFN-alpha/beta R1 Protein, CF

	Recombinant Cynomolgus Monkey IFN-alpha/beta R1 Protein Binding Activity. When Recombinant Cynomolgus Monkey IFN‑ alpha / beta R1 His-tags protein is immobilized at 10 µg/mL (100 µL/well), Recombinant Human IFN-A2 binds with an ED₅₀ of 1-6 ug/mL.
	Recombinant Cynomolgus Monkey IFN-alpha/beta R1 Protein SDS-PAGE. 2 μg/lane of Recombinant Cynomolgus Monkey IFN-alpha/beta R1 Protein, CF (Catalog # 10674-AB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-100 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10674-AB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-alpha/beta R1

Interferon-alpha/beta receptor 1 (IFN-alpha / beta R1), also known as IFNAR1, is a member of the class II cytokine receptor family of proteins. These proteins form heterodimeric receptor complexes that mediate class II cytokine signals and subunits of the different receptor complexes are shared and serve multiple functions (1). Mature human IFN-alpha / beta R1 consists of an extracellular domain (ECD) with three tandem fibronectin type III repeats, a transmembrane segment, and a cytoplasmic domain (2). Within the ECD, human IFN-alpha / beta R1 shares 47% and 50% amino acid sequence identity with mouse and rat IFN-alpha / beta R1, respectively. Alternative splicing generates two additional isoforms that lack the transmembrane segment and either all or a portion of the cytoplasmic domain. IFN-alpha / beta R1, in association with IFN-alpha / beta R2, is required for propagating anti-microbial signal transduction triggered by the type 1 interferons such as IFN-alpha and IFN-beta (3, 4). IFN-alpha / beta R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN-alpha / beta R2. Ligands preferentially associate with IFN-alpha / beta R2, and this complex subsequently forms a stable ternary assembly with IFN-alpha / beta R1 (5-7). IFN-alpha / beta R1 also associates with IFN-gamma R2 even in the absence of IFN-gamma stimulation (3). IFN-alpha / beta R1 activation depends on tyrosine phoshorylation as well as palmitoylation of its cytoplasmic domain (8, 9). Rapid down-regulation of the receptor is accomplished by ligand‑dependent or -independent pathways (e.g. VEGF R signaling, TLR signaling, or cellular stress) which induce its serine phosphorylation, ubiquitination, and degradation (10-13).

Item	Value
References	Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. Uze, G. et al. (1990) Cell 60:225. Hwang, S.Y. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11284. Takaoka, A. et al. (2000) Science 288:2357. Lamken, P. et al. (2004) J. Mol. Biol. 341:303. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867. Kalie, E. et al. (2008) J. Biol. Chem. 283:32925. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375. Claudinon, J. et al. (2009) J. Biol. Chem. 284:24328. Zheng, H. et al. (2011) Blood 118:4003. Qian, J. et al. (2011) PLoS Pathogens 7:e1002065. Bhattacharya, S. et al. (2010) J. Biol. Chem. 285:2318. Bhattacharya, S. et al. (2011) J. Biol. Chem. 286:22069.
Long Name	Interferon alpha/beta Receptor 1
Entrez Gene IDs	3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)
Alternate Names	AVP, CRF2-1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, IFN-R-1, IFN-aR1, IFN-alpha-REC, IFN-alpha/beta R1, IFN-alpha/beta receptor 1, IFN-bR1, IFNAR, IFNAR1, IFNBR, IFNbR1, Type I interferon receptor 1, alpha-type antiviral protein, beta-type antiviral protein, human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC, interferon (alpha, beta and omega) receptor 1, interferon alpha/beta receptor 1, interferon-alpha/beta receptor alpha chain, interferon-beta receptor 1

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Recombinant Cynomolgus Monkey IFN-alpha/beta R1 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Cynomolgus Monkey IFN-alpha/beta R1 Protein, CF

Scientific Data Examples for Recombinant Cynomolgus Monkey IFN-alpha/beta R1 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: IFN-alpha/beta R1

Citations for Recombinant Cynomolgus Monkey IFN-alpha/beta R1 Protein, CF

Product Documents for Recombinant Cynomolgus Monkey IFN-alpha/beta R1 Protein, CF

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