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Recombinant Cynomolgus Monkey GM-CSF R alpha His Protein, CF

Catalog # 10423-GR | R&D Systems, Inc. a Bio-Techne Brand
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10423-GR-050

Key Product Details

Accession #

Source

CHO

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey GM-CSF R alpha protein
Leu20-Arg313, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20

Predicted Molecular Mass

34 kDa

SDS-PAGE

59-67 kDa, under reducing conditions

Activity

Measured by its ability to inhibit GM-CSF-dependent proliferation of TF‑1 human erythroleukemic cells.
The ED50 for this effect is 1.0-5.0 μg/mL in the presence of 40 pg/mLRecombinant Human GM-CSF (Catalog # 215-GM).

Scientific Data Images for Recombinant Cynomolgus Monkey GM-CSF R alpha His Protein, CF

Recombinant Cynomolgus Monkey GM-CSF R alpha His Protein Inhibition Activity

Recombinant Cynomolgus Monkey GM-CSF R alpha His Protein Inhibition Activity

Measured by its ability to inhibit GM-CSF dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1.0-5.0 µg/mL in the presence of 40 pg/mL Recombinant Human GM-CSF (Catalog # 215-GM).
Recombinant Cynomolgus Monkey GM-CSF R alpha His Protein SDS-PAGE

Recombinant Cynomolgus Monkey GM-CSF R alpha His Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey GM-CSF R alpha His-tag (Catalog # 10423-GR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 59-67 kDa.

Formulation, Preparation and Storage

10423-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GM-CSF R alpha

Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1-4). Mature cynomolgus monkey GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of an extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a transmembrane segment and a cytoplasmic domain (5). Within the ECD, cynomolgus monkey GM-CSF R alpha shares approximately 90% and 84% amino acid sequence identity with human and rheus monkey GM-CSF R alpha, respectively. Alternate splicing of GM-CSF R alpha generates several additional isoforms that lack the cytoplasmic and/or transmembrane regions. Soluble forms of the receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non-hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( betac), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with betac converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9-11). The shared usage of betac underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).

References

  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
  3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
  4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
  5. Gearing, D.P. et al. (1989) EMBO J. 8:3667.
  6. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
  7. Raines, M.A. et al. (1991) Proc. Natl. Acad. Sci. 88:8203.
  8. Chiba, S. et al. (1990) Cell Regul. 1:327.
  9. Kitamura, T. et al. (1991) Proc. Natl. Acad. Sci. 88:5082.
  10. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
  11. Hoang, T. et al. (1993) J. Biol. Chem. 268:11881.

Long Name

Granulocyte Macrophage Growth Factor Receptor alpha

Alternate Names

CD116, CSF2R, CSF2RA, CSF2RY, GM-CSFRa, GMCSFR alpha

Entrez Gene IDs

1438 (Human); 12982 (Mouse); 102139775 (Cynomolgus Monkey)

Gene Symbol

CSF2RA

UniProt

Product Documents for Recombinant Cynomolgus Monkey GM-CSF R alpha His Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Monkey GM-CSF R alpha His Protein, CF

For research use only

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