Source	Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey GM-CSF R alpha protein Leu20-Arg313, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu20
Predicted Molecular Mass	34 kDa
SDS-PAGE	59-67 kDa, under reducing conditions
Activity	Measured by its ability to inhibit GM-CSF-dependent proliferation of TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 1.0-5.0 μg/mL in the presence of 40 pg/mLRecombinant Human GM-CSF (Catalog # 215-GM).

Recombinant Cynomolgus Monkey GM-CSF R alpha His Protein, CF Scientific Data Examples

	Recombinant Cynomolgus Monkey GM-CSF R alpha His Protein Inhibition Activity Measured by its ability to inhibit GM-CSF dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 1.0-5.0 µg/mL in the presence of 40 pg/mL Recombinant Human GM-CSF (Catalog # 215-GM).
	Recombinant Cynomolgus Monkey GM-CSF R alpha His Protein SDS-PAGE 2 μg/lane of Recombinant Cynomolgus Monkey GM-CSF R alpha His-tag (Catalog # 10423-GR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 59-67 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10423-GR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GM-CSF R alpha

Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1-4). Mature cynomolgus monkey GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of an extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a transmembrane segment and a cytoplasmic domain (5). Within the ECD, cynomolgus monkey GM-CSF R alpha shares approximately 90% and 84% amino acid sequence identity with human and rheus monkey GM-CSF R alpha, respectively. Alternate splicing of GM-CSF R alpha generates several additional isoforms that lack the cytoplasmic and/or transmembrane regions. Soluble forms of the receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non-hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9-11). The shared usage of beta c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).

Item	Value
References	Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163. Cao, Y. (2007) J. Clin. Invest. 117:2362. Gearing, D.P. et al. (1989) EMBO J. 8:3667. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483. Raines, M.A. et al. (1991) Proc. Natl. Acad. Sci. 88:8203. Chiba, S. et al. (1990) Cell Regul. 1:327. Kitamura, T. et al. (1991) Proc. Natl. Acad. Sci. 88:5082. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655. Hoang, T. et al. (1993) J. Biol. Chem. 268:11881.
Long Name	Granulocyte Macrophage Growth Factor Receptor alpha
Entrez Gene IDs	1438 (Human); 12982 (Mouse); 102139775 (Cynomolgus Monkey)
Alternate Names	CD116, CD116 antigen, CDw116, CSF2R, CSF2RA, CSF2RAX, CSF2RAY, CSF2RX, CSF2RY, GM-CSF R alpha, GM-CSF receptor alpha subunit, GM-CSF-R-alpha, GM-CSFRa, GMCSFR, GMCSFR alpha, GMCSFR-alpha, GMR, GMR-alpha, MGC3848, MGC4838, colony stimulating factor 2 receptor alpha subunit, colony stimulating factor 2 receptor, alpha, low-affinity(granulocyte-macrophage), granulocyte-macrophage colony-stimulating factor receptor alpha chain, granulocyte-macrophage colony-stimulating factor receptor subunit alpha

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Recombinant Cynomolgus Monkey GM-CSF R alpha His Protein, CF

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