Recombinant Cynomolgus CD2 Fc Chimera Protein, CF

Catalog # 10213-CD | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

Q6SZ61

Source

CHO

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD2 protein
Cynomolgus Monkey CD2
(Lys25-Asp209)
Accession # Q6SZ61		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys25

Predicted Molecular Mass

48 kDa

SDS-PAGE

60-66 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey CD2 Fc Chimera (Catalog # 10213-CD) is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human CD58/LFA-3 Fc Chimera (Catalog # 10068-CD) that produces 50% of the optimal binding response is 0.15-0.9 μg/mL.

Scientific Data Images for Recombinant Cynomolgus CD2 Fc Chimera Protein, CF

Recombinant Cynomolgus CD2 Fc Chimera Protein Binding Activity

Recombinant Cynomolgus CD2 Fc Chimera Protein Binding Activity

When Recombinant Cynomolgus CD2 Fc Chimera (Catalog # 10213-CD) is immobilized at 0.25 μg/mL, 100 μL/well, Recombinant Human CD58/LFA-3 Fc Chimera (Catalog # 10068-CD) binds with an ED50 of 0.15-0.9 μg/mL.
Recombinant Cynomolgus CD2 Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus CD2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey CD2 Fc Chimera (Catalog # 10213-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-66 kDa and 120-135 kDa, respectively.

Formulation, Preparation and Storage

10213-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD2

T cell surface antigen CD2 is a type I glycoprotein belonging to the Ig superfamily and is expressed on T cells, NK cells, B cells and some antigen presenting cells. CD2 functions as an activator of T lymphocytes and thymocytes (1). Mature human CD2 consists of a 185 amino acid (aa) extracellular domain (ECD) with an Ig-like V-type and an Ig-like C2 type domain, a 26 aa transmembrane segment, and a 116 aa cytoplasmic tail. The extracellular domain is composed of two immunoglobulin-superfamily domains with highly-charged binding regions (2). Within the ECD, Cynomolgus CD2 shares 92% aa sequence identity with human CD2. It interacts with both CD58 and CD59 directly to activate T cells and their adhesion pathways (3). The conformational flexibility of the CD2 molecule affects function through the conformational status of the adhesive ligands (4). Together with PSTPIP1, CD2 works to regulate T cell activation (5). These two proteins colocalize with a second CD2-binding protein to signal immunological synapse formation in T cells (6). CD2 is an adhesion molecule present on the cell surface of T cells, natural killer (NK) cells, and B cells; and its interaction with CD58 on antigen-presenting cells plays an important role in their immune reaction (7). CD2-CD58 interactions play a critical role in the anti-tumor immune response, and restoration of this signaling is an important strategy for anti-tumor therapy (8). Furthermore, CD2-CD58 interactions are pivotal for the activation and function of adaptive natural killer cells in human cytomegalovirus infection (9).

References

  1. Sayre, P. et al. (1987). Proc. Natl. Acad. Sci. 84:2941.
  2. Bodian, D.L. et al. (1994) Structure. 2:755.
  3. Hahn, W.C. et al. (1992). Science. 256:1805.
  4. Yang, J.J. et al. (2001). Curr. Prot. Pept. Sci. 2:1.
  5. Yang, H. and Reinherz, EL. (2006). J. Immunol. 176:5898.
  6. Badour, K. et al. (2003). Immunity. 18:141.
  7. Kingma, D.W. et al. (2002) Cytometry 50:243.
  8. Nishikori, M. et al. (2016) J Clin Cell Immunol 7:406.
  9. Rolle, A. et al. (2016) Eur. J. Immunol. 46:2420.

Alternate Names

CD2, LFA-2, T11

Entrez Gene IDs

914 (Human); 12481 (Mouse); 102120798 (Cynomolgus Monkey)

Gene Symbol

CD2

UniProt

Q6SZ61

Product Documents for Recombinant Cynomolgus CD2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Cynomolgus CD2 Fc Chimera Protein, CF

For research use only

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