Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF

Name: Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10068-CD
Price: 428 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10068-CD

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Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	P19256
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF

Product Specifications for Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human CD58/LFA-3 protein

Human CD58 (Phe29-Arg215) Accession # P19256	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe29

Predicted Molecular Mass

48 kDa

SDS-PAGE

71-82 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED₅₀ for this effect is 0.3-1.8 μg/mL.

Scientific Data Examples for Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF

	Recombinant Human CD58/LFA-3 Fc Chimera Protein Bioactivity Recombinant Human CD58 Fc Chimera (Catalog # 10068-CD) induces PHA-stimulated human T lymphoblasts proliferation. The ED₅₀ for this effect is 0.3-1.8 μg/mL.
	Recombinant Human CD58/LFA-3 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Human CD58/LFA‑3 Fc Chimera (Catalog# 10068-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 71-82 kDa and 140 -160 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10068-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD58/LFA-3

T cells require a signal induced by the engagement of the T cell receptor and a "co-stimulatory" signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Many cell-bound receptor-ligand pairs have now been shown to be involved in T cell co-stimulation including CD58/CD2 in humans and CD48/CD2 in mice and rats. CD58, also known as lymphocyte function-associated antigen (LFA-3), belongs to the CD2 family of the immunoglobulin superfamily (1). CD58 is widely expressed on hematopoietic and non-hematopoietic human tissue and has been found on leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts of human origin (2). No mouse or rat homolog of CD58 has as of yet been identified. CD58 has only one known ligand, CD2. CD2 is expressed on T cells, NK cells and dendritic cells (2-4). CD2 ligation by CD58 has been shown to mediate T cell adhesion, T cell activation, T cell cytokine production and T cell and NK cells cytotoxic activity (1, 3, 5, 6). In dendritic cells, CD2 engagement increases MHC Class II, CD40, CD80, CD86, CD58 and CCR7 and induces IL-1 beta and IL-12 cytokine secretion (4). Blockade of CD58-CD2 interaction on NK cells resulted in diminished production of IFN-gamma and TNF-alpha in response to Human cytomegalovirus (HCMV)-infected cells (7). Expression of CD58 was significantly increased in anisomycin-treated hepatocellular carcinoma (HCC), and may be critical player in NK-mediated immunotherapeutic effects (8).

Item	Value
References	Davis, S.J. and van der Merwe P.A. (1996) Immunol. Today 17:177. Smith, M.E. and J.A. Thomas (1990) J. Clin. Pathol. 43:893. Bolhuis, R.L. et al. (1986) J. Immunol. 136:3939. Crawford, K. et al. (2003) Blood 102:1745. Kanner, S.B. et al. (1992) J. Immunol. 148:2023. Bullens, D.M. et al. (2001) International Immunol. 13:181. Rölle A. et al. (2016) Eur J Immunol. 46:2420 Kim M. et al. (2018) Sci Rep. 8:10668.
Entrez Gene IDs	965 (Human); 102123839 (Cynomolgus Monkey); 710732 (Rhesus Macaque)
Alternate Names	Ag3, CD58, CD58 antigen, CD58 antigen, (lymphocyte function-associated antigen 3), CD58 molecule, FLJ23181, FLJ43722, LFA-3, LFA3, LFA3ag3, Surface glycoprotein LFA-3, lymphocyte function-associated antigen 3

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Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF

Scientific Data Examples for Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: CD58/LFA-3

Citations for Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF

Product Documents for Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF

Product Datasheets

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