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Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10068-CD

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10068-CD-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD58/LFA-3 protein
Human CD58
(Phe29-Arg215)
Accession # P19256
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe29

Predicted Molecular Mass

48 kDa

SDS-PAGE

71-82 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts.
The ED50 for this effect is 0.3-1.8 μg/mL.

Scientific Data Images for Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF

Recombinant Human CD58/LFA-3 Fc Chimera Protein Bioactivity

Recombinant Human CD58/LFA-3 Fc Chimera Protein Bioactivity

Recombinant Human CD58 Fc Chimera (Catalog # 10068-CD) induces PHA-stimulated human T lymphoblasts proliferation. The ED50 for this effect is 0.3-1.8 μg/mL.
Recombinant Human CD58/LFA-3 Fc Chimera Protein SDS-PAGE

Recombinant Human CD58/LFA-3 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CD58/LFA‑3 Fc Chimera (Catalog# 10068-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 71-82 kDa and 140 -160 kDa, respectively.

Formulation, Preparation and Storage

10068-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD58/LFA-3

T cells require a signal induced by the engagement of the T cell receptor and a "co-stimulatory" signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Many cell-bound receptor-ligand pairs have now been shown to be involved in T cell co-stimulation including CD58/CD2 in humans and CD48/CD2 in mice and rats. CD58, also known as lymphocyte function-associated antigen (LFA-3),  belongs to the CD2 family of the immunoglobulin superfamily (1). CD58 is widely expressed on hematopoietic and non-hematopoietic human tissue and has been found on leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts of human origin (2). No mouse or rat homolog of CD58 has as of yet been identified. CD58 has only one known ligand, CD2. CD2 is expressed on T cells, NK cells and dendritic cells (2-4). CD2 ligation by CD58 has been shown to mediate T cell adhesion, T cell activation, T cell cytokine production and T cell and NK cells cytotoxic activity (1, 3, 5, 6). In dendritic cells, CD2 engagement increases MHC Class II, CD40, CD80, CD86, CD58 and CCR7 and induces IL-1 beta and IL-12 cytokine secretion (4). Blockade of CD58-CD2 interaction on NK cells resulted in diminished production of IFN-gamma and TNF-alpha in response to Human cytomegalovirus (HCMV)-infected cells (7). Expression of CD58 was significantly increased in anisomycin-treated hepatocellular carcinoma (HCC), and may be critical player in NK-mediated immunotherapeutic effects (8).

References

  1. Davis, S.J. and van der Merwe P.A. (1996) Immunol. Today 17:177.
  2. Smith, M.E. and J.A. Thomas (1990) J. Clin. Pathol. 43:893.
  3. Bolhuis, R.L. et al. (1986) J. Immunol. 136:3939.
  4. Crawford, K. et al. (2003) Blood 102:1745.
  5. Kanner, S.B. et al. (1992) J. Immunol. 148:2023.
  6. Bullens, D.M. et al. (2001) International Immunol. 13:181.
  7. Rölle A. et al. (2016) Eur J Immunol. 46:2420
  8. Kim M. et al. (2018) Sci Rep. 8:10668.
 

Alternate Names

CD58, LFA-3, LFA3

Entrez Gene IDs

965 (Human); 102123839 (Cynomolgus Monkey); 710732 (Rhesus Macaque)

Gene Symbol

CD58

UniProt

Additional CD58/LFA-3 Products

Product Documents for Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

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