Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Aminopeptidase PILS/ARTS1 protein Ala37-Leu941 with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
95-107 kDa, under reducing conditions
Measured by its ability to cleave the fluorogenic peptide substrate, Leu-AMC. The specific activity is >800 pmol/min/μg, as measured under the described conditions.
Recombinant Cynomolgus Aminopeptidase PILS/ARTS1 Protein, CF Scientific Data Examples
Recombinant Cynomolgus Aminopeptidase PILS/ARTS1 Protein Enzyme Activity
Recombinant Cynomolgus Monkey Aminopeptidase PILS/ARTS1 His-tag (Catalog # 10237-ZN) is measured by its ability to cleave the fluorogenic peptide substrate, Leu-AMC.
Recombinant Cynomolgus Aminopeptidase PILS/ARTS1 Protein SDS-PAGE
2 μg/lane of Recombinant Cynomolgus Monkey Aminopeptidase PILS/ARTS1 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band under reducing conditions at 101 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
Substrate: Leu-AMC (Bachem, Catalog # I-1240), 10 mM stock in DMSO
F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
Dilute rcynoARTS1 to 1 µg/mL in Assay Buffer.
Dilute Substrate to 600 µM in Assay Buffer.
Load in plate 50 µL of 1 µg/mL rcynoARTS1, and start the reaction by adding 50 µL of 600 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 600 µM Substrate.
Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode of 5 minutes.
Calculate specific activity:
Specific Activity (pmol/min/µg) =
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)
*Adjusted for Substrate Blank **Derived using calibration standard 7-amino, 4-Methyl Coumarin AMC (Sigma, Catalog # A9891)
rcynoARTS1: 0.05 µg
Substrate: 300 µM
Background: Aminopeptidase PILS/ARTS1
ARTS1 encodes a zinc-dependent metalloprotease (1) known as aminopeptidase PILS (Puromycin-Insensitive Leucyl-Specific), ALAP (adipocyte-derived leucine aminopeptidase), TNFR (type 1 tumor necrosis factor receptor) shedding aminopeptidase regulator, or ERAP1 (endoplasmic reticulum aminopeptidase 1). ARTS1 is widely expressed and known to be present in the endoplasmic reticulum. The protein has four domains with a large cavity between domain II and IV that can accommodate large peptide substrates; the second domain contains the active site and the first domain caps the active site and provides binding sites for the amino terminus of the substrate peptides (2). The enzyme has a unique substrate preference amongst the M1-family of aminopeptidases for longer peptides of up to 16 residues (3, 4) that is consistent with its ability to trim antigen precursors for MHC class 1 presentation (5). Variants and polymorphisms identified in ARTS-1 have linked it to autoimmune disease (6), cancer (7), and hypertension (8). ARTS-1 has been shown to be secreted (9, 10) and cause activation of inflammasome and cathepsin B pathways (11) and consequently to have a direct role in innate immunity and contribute to autoinflammatory and autoimmune diseases (12). ARTS-1 is consequently a potential therapeutic target (12-14).
Schomburg, L. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 311, Academic Press, San Diego.
Nguyen, T. T. et al. (2011) Nat. Struct. Mol. Biol. 18:604.
York, I. A. et al. (2002) Nat. Immunol. 3:1177.
Chang, S. C. et al. (2005) Proc. Nat. Acad. Sci. U.S.A. 102:17107.
Serwold, T. et al. (2002) Nature 419:480.
Fung, E. Y. et al. (2009) Genes Immun. 10:188.
Mehta, A. M. et al. (2009) Genes Chromosomes Cancer 48:410.
Yamamoto, N. et al. (2002) Hum. Mutat. 19:251.
Cui, X. et al. (2002) J. Clin. Invest. 110:515.
Goto, Y. et al. (2011) J. Biol. Chem. 286:21906.
Aldhamen, Y. A. et al. (2015) J. Innate Immun. 7:275.
Pepelyayeva, Y. and A. Amalfitano. (2019) Hum. Immunol. 80:302.
Koumantou, D. et al. (2019) Cancer Immunol. Immunother. 68:1245.
Georgiadis, D. et al. (2018) Curr. Med. Chem. [Epub ahead of print]
Which Brands are Currently Available on bio-techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments and Simple Plex Assays are available to quote. ProteinSimple branded instrument consumables and ACD branded products will be available on bio-techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
Setting the standard in quality research reagents for over 30 years
A trusted leader in quality life science reagents
Your trusted supplier for innovative and high performance life science reagents
Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis
Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity
A world leader in developing liquid biopsy based diagnostics