Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >2000 pmol/min/μg, as measured under the described conditions.
Recombinant Cyno u-Plasminogen Activator (uPA) Protein, CF Scientific Data Examples
Recombinant Cynomolgus Monkey u‑Plasminogen Activator (uPA)/Urokinase His-tag Protein Enzyme Activity.
Recombinant Cynomolgous Monkey u-Plasminogen Activator (uPA)/Urokinase His-tag (Catalog # 11098-SE) is measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC).
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in HEPES, NaCl and CaCl2.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
Urokinase Plasminogen Activator (uPA), also known as u-plasminogen activator or urokinase, is a highly-specific serine protease from the peptidase S1 family that cleaves plasminogen to form plasmin making it a key player in the plasminogen activator (PA) system (1, 2). In cancer, the PA system plays a commanding role in tumor growth, angiogenesis, tumor cell invasion, migration, and metastasis. Expression of uPA is minimal in normal cells but is increased several fold in tumor cells by extracellular stimuli elevated in cancer (3) and corresponds to poor outcomes in several types of cancer (2, 4-7). Therefore, uPA has been identified as an excellent target for therapeutic development through inhibition of protease activity or though inhibition of uPA-dependent signaling while in complex with uPA receptor (uPAR) (2, 7). The pro-enzyme of uPA is synthesized with an N-terminal signal peptide and processed into an active disulfide-linked two-chain molecule (2, 7-10). For the cynomolgous uPA, the B chain starting at Ile178 corresponds to the catalytic domain. Two forms of the A chain exist, one starting at Ser21 (the long form) and the other at Lys155 (the short form). While the B chain is common for both forms, the long and short A chains are unique to expected 48 kDa and 32 kDa two-chain forms, respectively. The long A chain contains an EGF-like domain and the kringle domain. The long A domain is reportedly responsible for the binding of the uPA receptor (uPAR) (2,7). Both high and low MW forms exist in the purified recombinant cyno uPA.
Ellis, V. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. Academic Press, San Diego, pp.1677.
Mahmood, N. et al. (2018) Front Oncol. 8:24.
Nagamine, Y. et al. (2005) Thromb. Haemost. 93:661.
Duffy, M. and C. Duggan (2004) Clin. Biochem. 37:541.
Pappot, H. et al. (2006) Lung Cancer 51:193.
Taubert, H. et al. (2010) Br. J. Cancer 102:731.
Masucci, M.T. et al. (2022) Cancers. 14:498.
Riccio, A. et al. (1985) Nucleic Acids Res. 13:2759.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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