Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF

Name: Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF
Brand: R&D Systems
SKU: 10071-AB
Price: 394 USD

Catalog # 10071-AB | R&D Systems, Inc. a Bio-Techne Brand

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	XP_005548871.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF

Product Specifications for Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived IFN-alpha/beta R2 protein

Cyno/Rhesus IFN-alpha R2 (Ile 27-Lys243) Accession # XP_005548871.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ile 27

Predicted Molecular Mass

51.4 kDa

SDS-PAGE

75 - 95 kDa, reducing conditions

Activity

Measured by its ability to inhibit Type-I IFN-mediated anti-viral activity. The ED₅₀ for this effect is 0.500-3.50 μg/mL in the presence of 30 pg/mL of Recombinant Human IFN‑ beta (Catalog # 8499-IF).

Scientific Data Examples for Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF

	Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein Inhibition Activity Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Chimera (Catalog # 10071-AB) inhibits the anti-viral activity of (Catalog # 8499-IF) The ED₅₀ for this effect is 0.500-3.50 µg/mL.
	Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein SDS-PAGE 2 μg/lane of Recombinant Cyno/Rhesus IFN‑ alpha / beta R2 Fc Chimera (Catalog # 10071-AB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-95 kDa and 150-190 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10071-AB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-alpha/beta R2

IFN-alpha/beta R2, also known as IFNAR2, is a 100 kDa glycoprotein in the class II cytokine receptor family. These proteins form heterodimeric receptor complexes that transduce signals from the interferon, IL-10, and IL-28 families of cytokines (1, 2). Mature IFN-alpha / beta R2 consists of an extracellular domain (ECD), containing two fibronectin type III repeats, a transmembrane segment, and a cytoplasmic domain. Alternative splicing generates a secreted isoform that corresponds to the ECD as well as a 50 kDa transmembrane isoform with a substituted and truncated cytoplasmic region (3, 4). The short isoform is impaired in its ability to activate signaling molecules and functions as a dominant negative receptor subunit (5-7). The mature ECD of cynomolgus IFN-alpha/ beta R2 shares 93% and 100% amino acid (aa) sequence identity with that of human and rhesus, respectively. IFN-alpha/beta R2, in association with IFN-alpha/beta R1, is required for mediating the antiviral, antiproliferative, and apoptotic effects of the type I interferons IFN-alpha and IFN-beta. IFN-alpha / beta R2 is the principal ligand binding subunit of the receptor. Ligand binding is stabilized by the subsequent association with IFN-alpha / beta R1, resulting in the formation of a signaling ternary receptor complex (8, 9). IFN-alpha / beta R2 is also subject to presenilin-dependent intramembrane proteolysis, resulting in the liberation of nearly the entire ECD as well as the cytoplasmic domain which migrates to the nucleus and can inhibit gene transcription (10). High concentrations of soluble IFN-alpha / beta R2 bind and neutralize IFN-alpha and IFN-beta, while lower concentrations prolong the antiviral activity of circulating IFN-beta but not IFN-alpha (11). Human but not mouse IFN-alpha / beta R2 constitutively associates with STAT4, which may account for species specific differences observed in type I interferon responses (12).

Item	Value
References	Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. Pestka, S. et al. (2004) Immunol. Rev. 202:8. Lutfalla, G. et al. (1995) EMBO J. 14:5100. Novick, D. et al. (1995) J. Leukocyte Biol. 57:712. Pfeffer, L.M. et al. (1997) J. Biol. Chem. 272:11002. Gazziola, C. et al. (2005) Int. J. Oncol. 26:129. Kotenko, S.V. and S. Pestka (2000) Oncogene 19:2557. Lamken, P. et al. (2004) J. Mol. Biol. 341:303. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867. Saleh, A.Z.M. et al. (2004) Oncogene 23:7076. McKenna, S.D. et al. (2004) J. Interferon Cytokine Res. 24:119. Tyler, D.R. et al. (2007) Mol. Immunol. 44:1864.
Long Name	Interferon alpha/beta Receptor 2
Entrez Gene IDs	3455 (Human); 15976 (Mouse); 102140083 (Cynomolgus Monkey)
Alternate Names	IFN-R, IFN-R-2, IFN-aR2, IFN-alpha binding protein, IFN-alpha-REC, IFN-alpha/beta R2, IFN-alpha/beta receptor 2, IFN-bR2, IFNABR, IFNAR2, IFNARB, IFNbR2, Interferon alpha binding protein, Type I interferon receptor 2, beta receptor10, human interferon alpha, human interferon alpha/beta receptor, interferon (alpha, beta and omega) receptor 2, interferon alpha/beta receptor 2, interferon-alpha/beta receptor beta chain

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Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF

Catalog # 10071-AB | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF

Scientific Data Examples for Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: IFN-alpha/beta R2

Citations for Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF

Product Documents for Recombinant Cyno/Rhesus IFN-alpha/beta R2 Fc Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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