Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Lymphotoxin beta R/TNFRSF3 protein Ser28-Met227, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Ser28 & Gln29 & Gln 31
Predicted Molecular Mass
29-41 kDa, under reducing conditions.
Measured by its ability to inhibit Recombinant Human Lymphotoxin alpha 1/ beta 2 (Catalog # 8884-LY) induced IL-8 secretion in A375 human melanoma cells. The ED50 for this effect is 0.125-1.50 μg/mL.
Recombinant Cyno Lymphotoxin beta R/TNFRSF3 His Protein, CF Scientific Data Examples
Recombinant Cynomolgus Monkey Lymphotoxin beta R/TNFRSF3 His-tag Protein Bioactivity.
Recombinant Cynomolgus Monkey Lymphotoxin beta R/TNFRSF3 His-tag Protein (Catalog # 11152-LR) inhibits Recombinant Human Lymphotoxin alpha 1/ beta 2 (8884-LY) induced IL-8 secretion in A375 human melanoma cells. The ED50 for this effect is 0.125-1.50 μg/mL.
Recombinant Cynomolgus Monkey Lymphotoxin beta R/TNFRSF3 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Cynomolgus Monkey Lymphotoxin beta R/TNFRSF3 His-tag Protein (Catalog # 11152-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 29-41 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lymphotoxin beta R/TNFRSF3
Lymphotoxin beta receptor (LT beta R), previously called TNF RIII or TNF R‑related protein (TNF Rrp), is a type I transmembrane glycoprotein within the TNF receptor superfamily, designated TNFRSF3 (1‑3). Cynomolgus monkey and rhesus macaque LT beta R cDNA encodes 435 amino acids (aa) including a 30 aa signal peptide, a 197 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 187 aa cytoplasmic domain. The ECD contains four cysteine‑rich motifs characteristic of the TNF receptor superfamily (1, 2). Within the ECD cynomolgus monkey LTBR shares 65‑75% aa sequence identity with human, mouse, rat, canine, porcine, equine and bovine LT beta R. Soluble LT beta R can be formed by proteolytic cleavage of the ECD, and is an inhibitor of transmembrane LT beta R, as is recombinant LT beta R, which inhibits autoimmunity (3‑6). LT beta R is expressed by visceral, lymphoid, and other stroma, epithelia and myeloid cells, but not lymphocytes (2, 4). LT beta R ligands include homotrimers of LIGHT (TNFSF14; also a ligand for HVEM) and the heterotrimeric lymphotoxin LT alpha 1/ beta 2 (3, 4, 6). Depending on the cell type and expression of TRAF3, activation of LT beta R has been shown to induce canonical (IKK/RelA; pro‑inflammatory) or alternative (NIK/RelB; lymphoid organogenic) NF kappa B activation (6, 7). LT beta R is expressed on mesenchymal stromal organizing cells that give rise to stroma of primary (thymus), secondary (tonsils, lymph nodes and Peyers patches) and tertiary (ectopic inflammatory) lymphoid structures (3‑5, 8‑10). Secondary immune tissues are absent in LT beta R‑deficient mice (3-5). LT beta R engagement induces production of IL‑7, RANK, TRANCE/RANK L, VEGF‑C, adhesion molecules such as VCAM‑1, ICAM‑1 and MAdCAM, and chemokines such as CXCL13, CCL19 and CCL21 (3, 8 ‑ 10). LT beta R is expressed by hepatocytes, is up‑regulated in regeneration, hepatitis and hepatocellular carcinoma, and influences lipid metabolism and atherosclerosis (4, 6, 11). It regulates cell growth and can initiate inflammation‑related carcinogenesis (6, 11).
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Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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