Recombinant Cotton Rat CXCL10/IP-10 Protein, CF

Name: Recombinant Cotton Rat CXCL10/IP-10 Protein, CF
Brand: R&D Systems
SKU: 1117-IP

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1117-IP

Citations (2)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	AAL16935
Applications:	Bioactivity

View Full Technical Details for Recombinant Cotton Rat CXCL10/IP-10 Protein, CF

Product Specifications for Recombinant Cotton Rat CXCL10/IP-10 Protein, CF

Source	E. coli-derived cotton rat CXCL10/IP-10/CRG-2 protein Ile22-Val94, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	8.6 kDa
Activity	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CXCR3. The ED₅₀ for this effect is 2-10 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1117-IP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CXCL10/IP-10/CRG-2

P-10, also named mob-1 in rat and crg-2 in mouse, is a member of the non-ELR-motif containing CXC chemokine subfamily and has been designated CXCL10. It is induced in various cell types (including keratinocytes, endothelial cells, fibroblasts, smooth muscle cells, monocytes/macrophages, astrocytes, neutrophils, hepatocytes, T cells, epithelial cells and mesothelial cells) in response to stimulation by type I and/or type II interferon as well as by LPS. IP-10 has been shown to chemoattract activated CD4+ and CD8+ T cells, activated NK cells and smooth muscle cells. It also induces the adhesion of activated T cells to VCAM and ICAM. In addition, IP-10 has been reported to antagonize the angiogenic activity of FGF. IP-10 binds to and activates CXCR3, the chemokine receptor that is shared by MIG/CXCL9 and I-TAC/CXCL11, two additional non-ELR CXC chemokines that are induced by interferon. Cotton rat IP-10 cDNA encodes a 94 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 73 aa mature protein with one potential N-linked glycosylation site. Mature cotton rat IP-10 shares 66%, 64%, and 66% aa sequence identity with rat, mouse and human IP10, respectively (1 - 7).

Item	Value
References	Vanguri, P. and J.M. Farber (1990) J. Biol. Chem. 265:15049. Liang, P. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:12515. Proost, P. et al. (1993) J. Immunol. 150:1000. Blanco, J.C. et al. (2001) GenBank Accession # AAL16959. Neville, L.F. (1997) Prog. Growth Factor Res. 8:207. Belperio, J.A. et al. (2000) J. Leukoc. Biol. 68:1. Soejima, K. and B.J. Rollins (2001) J. Immunol. 167:6576.
Entrez Gene IDs	3627 (Human); 15945 (Mouse)
Alternate Names	C7, CRG-2, CRG2, CXCL10, IFI10, INP10, IP-10, SCYB10, chemokine (C-X-C motif) ligand 10, gIP-10, mob-1

Citations for Recombinant Cotton Rat CXCL10/IP-10 Protein, CF (2)

Showing 1 - 2 of 2 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Cotton Rat CXCL10/IP-10 Protein, CF include:

Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Nathalie Strazielle et al. (2016), T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium. PLoS ONE, 0(0):1932-6203.
PMID: 26942913
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Barkho BZ et al. (2006), Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation. Stem Cells Dev., 15(3):407-21.
PMID: 16846377

There are no citations that match your criteria.

Showing 1 - 2 of 2 citations Showing All

Product Documents for Recombinant Cotton Rat CXCL10/IP-10 Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Recombinant Cotton Rat CXCL10/IP-10 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased