Recombinant Cotton Rat CCL4/MIP-1 beta Protein

Name: Recombinant Cotton Rat CCL4/MIP-1 beta Protein
Brand: R&D Systems
SKU: 1024-MB

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1024-MB

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Key Product Details

Source:	E. coli
Accession #:	AAL16933
Applications:	Bioactivity

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Product Specifications for Recombinant Cotton Rat CCL4/MIP-1 beta Protein

Source	E. coli-derived cotton rat CCL4/MIP-1 beta protein Ala24-Asn92
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala24
Predicted Molecular Mass	7.8 kDa
Activity	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR5. The ED₅₀ for this effect is 1-5 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1024-MB

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1024-MB/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL4/MIP-1 beta

CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta ), is a 12 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium (3 - 8). A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment (6). CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha (9). The first two N-terminal amino acids (aa) can be cleaved from human CCL4 by CD26/DPPIV (10, 11). Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2 (10). In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor (2). Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5 (10). Mature cotton rat CCL4 shares 75% - 83% aa sequence identity with human, mouse, and rat CCL4.

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References	Rot, A. and U.H. von Andrian (2004) Annu. Rev. Immunol. 22:891. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455. Sun, X. et al. (2006) Infec. Immun. 74:5943. Bisset, L.R. and P. Schmid-Grendelmeier (2005) Curr. Opin. Pulm. Med. 11:35. Frangogiannis, N.G. (2004) Inflamm. Res. 53:585. Bystry, R.S. et al. (2001) Nat. Immunol. 2:1126. Oliveira, S.H.P. et al. (2002) J. Leukoc. Biol. 71:1019. Schall, T.J. et al. (1993) J. Exp. Med. 177:1821. Guan, E. et al. (2001) J. Biol. Chem. 276:12404. Guan, E. et al. (2002) J. Biol. Chem. 277:32348. Guan, E. et al. (2004) J. Cell. Biochem. 92:53.
Entrez Gene IDs	6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine); 102117861 (Cynomolgus Monkey)
Alternate Names	ACT-2, ACT2, AT744.1, C-C motif chemokine 4, CCL4, Exodus-3, G-26, G-26 T-lymphocyte-secreted protein, HC21, LAG-1, LAG1, Lymphocyte activation gene 1 protein, MIP-1 beta, MIP-1-beta, MIP-1-beta(1-69), MIP1 beta, MIP1B, MIP1B1, Macrophage inflammatory protein 1-beta, PAT 744, Protein H400, SCYA2, SCYA4, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2, chemokine (C-C motif) ligand 4, secreted protein G-26, small inducible cytokine A4 (homologous to mouse Mip-1b)

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Recombinant Cotton Rat CCL4/MIP-1 beta Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Cotton Rat CCL4/MIP-1 beta Protein

Formulation, Preparation and Storage

Carrier Free

1024-MB

Carrier

1024-MB/CF

Carrier Free

Reconstitution Calculator

Background: CCL4/MIP-1 beta

Citations for Recombinant Cotton Rat CCL4/MIP-1 beta Protein

Product Documents for Recombinant Cotton Rat CCL4/MIP-1 beta Protein

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