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Recombinant Cotton Rat CCL3/MIP-1 alpha Protein

Catalog # 1061-RM | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
1061-RM-025/CF

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With Carrier
1061-RM-025

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived cotton rat CCL3/MIP-1 alpha protein
Ala24-Ala92

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala24

Predicted Molecular Mass

7.9 kDa

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5.
The ED50 for this effect is 1.5-6 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1061-RM
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1061-RM/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL3/MIP-1 alpha

CCL3, also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha) and LD78, is a member of the beta or CC subfamily of chemokines and is closely related to CCL4/MIP-1 beta. Chemokines comprise a large family of small secreted proteins that are involved in immune and inflammatory responses. CCL3 expression can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells (1). Mature cotton rat CCL3 shares 70%, 82%, and 88% amino acid sequence identity with human, mouse, and rat CCL3, respectively. CCL3 is an approximately 8 kDa chemokine that forms complexes with sulfated proteoglycans (2, 3). In a reversible process, CCL3 associates into noncovalently-linked dimers which then form tetramers and high molecular weight polymers (4, 5). These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine (5). CCL3 exerts its biological functions through interactions with CCR1, CCR3, and CCR5 (1). It is cleared from the extracellular space by internalization via the decoy chemokine receptor D6 (6). CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils (1, 7 - 9). CCL3 is also known as stem cell inhibitor (SCI) and can inhibit the proliferation of hematopoietic progenitor cells (2). CCL3 bioactivity contributes to tumor metastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis (10 - 13), although it also can suppress the replication of HIV (14). CCL3 additionally promotes hyperalgesia by sensitizing sensory neurons to TRPV1-mediated noxious stimulation (15).

References

  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
  2. Graham, G.J. et al. (1990) Nature 344:442.
  3. Wagner, L. et al. (1998) Nature 391:908.
  4. Graham, G.J. et al. (1994) J. Biol. Chem. 269:4974.
  5. Ren, M. et al. (2010) EMBO J. 29:3952.
  6. Weber, M. et al. (2004) Mol. Biol. Cell 15:2492
  7. Taub, D.D. et al. (1993) Science 260:355.
  8. Bernardini, G. et al. (2008) Blood 111:3626.
  9. Lee, S.C. et al. (2000) J. Immunol. 164:3392.
  10. Wu, Y. et al. (2008) J. Immunol. 181:6384.
  11. Cook, D.N. et al. (1995) Science 269:1583.
  12. Chintalacharuvu, S.R. et al. (2005) Immunol. Lett. 100:202.
  13. Ajuebor, M.N. et al. (2004) Eur. J. Immunol. 34:2907.
  14. Cocchi, F. et al. (1995) Science 270:1811.
  15. Zhang, N. et al. (2005) Proc. Natl. Acad. Sci. 102:4536.

    Alternate Names

    G0S19-1, LD78a, MIP-1 alpha, MIP1 alpha, MIP1A, PAT 464.1, SCYA3, SIS-beta

    Entrez Gene IDs

    6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)

    Gene Symbol

    CCL3

    UniProt

    Additional CCL3/MIP-1 alpha Products

    Product Documents for Recombinant Cotton Rat CCL3/MIP-1 alpha Protein

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Product Specific Notices for Recombinant Cotton Rat CCL3/MIP-1 alpha Protein

    For research use only

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