Recombinant Cotton Rat CCL3/MIP-1 alpha Protein

Name: Recombinant Cotton Rat CCL3/MIP-1 alpha Protein
Brand: R&D Systems
SKU: 1061-RM

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1061-RM

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Key Product Details

Source:	E. coli
Accession #:	AAL26704
Applications:	Bioactivity

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Product Specifications for Recombinant Cotton Rat CCL3/MIP-1 alpha Protein

Source	E. coli-derived cotton rat CCL3/MIP-1 alpha protein Ala24-Ala92
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala24
Predicted Molecular Mass	7.9 kDa
Activity	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED₅₀ for this effect is 1.5-6 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1061-RM

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1061-RM/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL3/MIP-1 alpha

CCL3, also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha ) and LD78, is a member of the beta or CC subfamily of chemokines and is closely related to CCL4/MIP-1 beta. Chemokines comprise a large family of small secreted proteins that are involved in immune and inflammatory responses. CCL3 expression can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells (1). Mature cotton rat CCL3 shares 70%, 82%, and 88% amino acid sequence identity with human, mouse, and rat CCL3, respectively. CCL3 is an approximately 8 kDa chemokine that forms complexes with sulfated proteoglycans (2, 3). In a reversible process, CCL3 associates into noncovalently-linked dimers which then form tetramers and high molecular weight polymers (4, 5). These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine (5). CCL3 exerts its biological functions through interactions with CCR1, CCR3, and CCR5 (1). It is cleared from the extracellular space by internalization via the decoy chemokine receptor D6 (6). CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils (1, 7 - 9). CCL3 is also known as stem cell inhibitor (SCI) and can inhibit the proliferation of hematopoietic progenitor cells (2). CCL3 bioactivity contributes to tumor metastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis (10 - 13), although it also can suppress the replication of HIV (14). CCL3 additionally promotes hyperalgesia by sensitizing sensory neurons to TRPV1-mediated noxious stimulation (15).

Item	Value
References	Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455. Graham, G.J. et al. (1990) Nature 344:442. Wagner, L. et al. (1998) Nature 391:908. Graham, G.J. et al. (1994) J. Biol. Chem. 269:4974. Ren, M. et al. (2010) EMBO J. 29:3952. Weber, M. et al. (2004) Mol. Biol. Cell 15:2492 Taub, D.D. et al. (1993) Science 260:355. Bernardini, G. et al. (2008) Blood 111:3626. Lee, S.C. et al. (2000) J. Immunol. 164:3392. Wu, Y. et al. (2008) J. Immunol. 181:6384. Cook, D.N. et al. (1995) Science 269:1583. Chintalacharuvu, S.R. et al. (2005) Immunol. Lett. 100:202. Ajuebor, M.N. et al. (2004) Eur. J. Immunol. 34:2907. Cocchi, F. et al. (1995) Science 270:1811. Zhang, N. et al. (2005) Proc. Natl. Acad. Sci. 102:4536.
Entrez Gene IDs	6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)
Alternate Names	C-C motif chemokine 3, MIP1-(a), AI323804, CCL3, G0S19-1, LD78a, LD78alpha, MIP-1 alpha, MIP-1alpha, MIP1 alpha, MIP1-alpha, MIP1A, PAT 464.1, SCYA3, SIS-beta, chemokine (C-C motif) ligand 3

Citations for Recombinant Cotton Rat CCL3/MIP-1 alpha Protein

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Recombinant Cotton Rat CCL3/MIP-1 alpha Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Cotton Rat CCL3/MIP-1 alpha Protein

Formulation, Preparation and Storage

Carrier Free

1061-RM

Carrier

1061-RM/CF

Carrier Free

Reconstitution Calculator

Background: CCL3/MIP-1 alpha

Citations for Recombinant Cotton Rat CCL3/MIP-1 alpha Protein

Product Documents for Recombinant Cotton Rat CCL3/MIP-1 alpha Protein

Product Datasheets

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