Key Product Details
Structure / Form
(Asp16 - Asn272) and
(Glu18 - Asn272)
Accession # NP_990154
(Pro100 - Lys330)
N-terminal Sequence Analysis
Predicted Molecular Mass
80 ng per embryo induces 25-50% heart reversals.
Measured by its binding ability in a functional ELISA.
Immobilized rchCaronte/Fc Chimera at 5 µg/mL (100 µL/well) can bind hBMP-4 with a linear range of 0.8-100 ng/mL.
Formulation, Preparation and Storage
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
Reconstitute at 100 μg/mL in sterile PBS.
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Chicken Caronte, the ortholog of mammalian Cerberus, is a member of the Cerberus/DAN family of secreted glycoproteins. There are at least 5 Dan Domain (DAND) family members including DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), Cerberus (DAND4), and Coco (DAND5). Caronte is expressed in the left paraxial mesoderm and is the molecule responsible for transducing left-sided positional information from the node to the periphery of the lateral plate mesoderm (LPM) during chicken embryonic development. Caronte functions as a BMP antagonist, inducing Nodal and downstream genes in the LPM by interfering with the repressive activity of bilaterally-produced BMPs. In addition, antagonism of BMPs by Caronte induces the expression of the Lefty gene in the midline. Caronte binds both BMP-4 and BMP-7 in immunoprecipitation experiments. Recombinant chicken Caronte preparations from R&D Systems have been shown to bind BMP-4 in a functional ELISA and induce heart reversals in Xenopus embryos.
- Pearce, J. et al. (1999) Dev. Biol. 209:98.
- Yokouchi, Y. et al. (1999) Cell. 98:573.
- King, T. and N. Brown (1999) Nature 401:222.
- Ahu, L. et al. (1999) Curr. Biol. 9:931.
- Rodriguez-Esteban, C. et al. (1999) Nature 401:243.
Entrez Gene IDs
Product Specific Notices for Recombinant Chicken Caronte Fc Chimera Protein, CF
For research use only