Recombinant Canine IFN-gamma Protein

Name: Recombinant Canine IFN-gamma Protein
Brand: R&D Systems
SKU: 781-CG

Catalog # 781-CG | R&D Systems, Inc. a Bio-Techne Brand

Citations (9)

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Key Product Details

Source:	E. coli
Accession #:	P42161
Applications:	Bioactivity

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Technical Data
Citations (9) / Reviews

Product Specifications for Recombinant Canine IFN-gamma Protein

Source	E. coli-derived canine IFN-gamma protein Gln24-Lys166, with an N-terminal Met
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	17 kDa
SDS-PAGE	17 kDa
Activity	Measured by its anti-viral activity on A‑72 canine fibroma cells infected with vesicular stomatitis virus (VSV). The ED₅₀ for this effect is 0.3-1.5 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

781-CG

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

781-CG/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature canine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 79%‑88% amino acid sequence identity with bovine, equine, and feline IFN-gamma, 62%‑73% with human, porcine, and rhesus IFN-gamma, and 40%-47% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

Item	Value
References	Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. Pestka, S. et al. (2004) Immunol. Rev. 202:8. Zucker, K. et al. (1992) J. Interferon Res. 12:191. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names	IFG, IFI, IFN-gamma, IFNG, IFNgamma, Immune interferon, interferon gamma, interferon, gamma

Citations for Recombinant Canine IFN-gamma Protein (9)

Showing 1 - 9 of 9 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Canine IFN-gamma Protein include:

Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

M Pelst et al. (2022-09-13), Immortalised canine buccal epithelial cells' CXCL8 secretion is affected by allergen extracts, Toll-like receptor ligands, IL-17A and calcitriol Veterinary research, 2022-053(1):72.
PMID: 36100942
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

JW Choi et al. (2020-07-02), Development of canine PD-1/PD-L1 specific monoclonal antibodies and amplification of canine T cell function PLoS ONE, 2020-015(7):e0235518.
PMID: 32614928
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

KA Lahey et al. (2017-07-03), Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function PLoS ONE, 2017-012(7):e0180259.
PMID: 28671992
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

C Westenfeld et al. (2017-05-03), Durable Control of Autoimmune Diabetes in Mice Achieved by Intraperitoneal Transplantation of Neo-Islets, Three-Dimensional Aggregates of Allogeneic Islet and Mesenchymal Stem Cells Stem Cells Transl Med, 2017-00(0).
PMID: 28467694
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

(2016-12-22), Suppression of Canine Dendritic Cell Activation/Maturation and Inflammatory Cytokine Release by Mesenchymal Stem Cells Occurs Via Multiple Distinct Biochemical Pathways Stem Cells Dev., 2016-10(0).
PMID: 27842458
Species: Canine
Sample Types: Recombinant Protein
Applications: ELISA (Standard)

(2016-05-25), Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs PLoS Negl Trop Dis, 2016-010(5):e0004614.
PMID: 27223609
Sample Types: N/A
Applications: ELISA (Standard)

Lemesre JL et al. (2007-03-15), Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France: double-blind randomised efficacy field trial. Vaccine, 2007-025(21):4223-34.
PMID: 17395339
Sample Types: N/A
Applications: ELISA (Standard)

Fujiwara RT et al. (2005-08-15), Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs. Vaccine, 2005-024(4):501-9.
PMID: 16140437
Sample Types: N/A
Applications: ELISA (Standard)

Lemesre JL et al. (2005-04-22), Protection against experimental visceral leishmaniasis infection in dogs immunized with purified excreted secreted antigens of Leishmania infantum promastigotes. Vaccine, 2005-023(22):2825-40.
PMID: 15780731

Recombinant Canine IFN-gamma Protein

Catalog # 781-CG | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Canine IFN-gamma Protein

Formulation, Preparation and Storage

Carrier Free

781-CG

Carrier

781-CG/CF

Carrier Free

Reconstitution Calculator

Background: IFN-gamma

Citations for Recombinant Canine IFN-gamma Protein (9)

Product Documents for Recombinant Canine IFN-gamma Protein

Product Datasheets

COA

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SDS

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