What receptors does FGF basic bind?

Name: Recombinant Canine FGF basic/FGF2/bFGF Protein
Brand: R&D Systems
SKU: 8575-FB

FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.rndsystems.com/resources/articles/fibroblast-growth-factors-and-their-receptors

Recombinant Canine FGF basic/FGF2/bFGF Protein

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 8575-FB

Citations (1)

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Source:	E. coli
Accession #:	FIP6K6
Applications:	Bioactivity

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Product Specifications for Recombinant Canine FGF basic/FGF2/bFGF Protein

Source	E. coli-derived canine FGF basic/FGF2/bFGF protein Pro10-Ser155 (Lys36Asn)
Purity	>95%, by SDS-PAGE with silver staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Pro10 & Ala11
Predicted Molecular Mass	16 kDa
SDS-PAGE	17 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 0.2-1 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

8575-FB

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄, TCEP and EDTA with BSA as a carrier protein.
Reconstitution	Reconstitute at 250 μg/mL in water containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8575-FB/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄, TCEP and EDTA.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF basic/FGF2/bFGF

FGF basic, also known as FGF-2 or HBGF-2, is a widely expressed member of the fibroblast growth factor family (1-3). FGF basic functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain (2, 4-6). It is up-regulated in response to inflammatory stimuli such as TNF-alpha, IL-1 beta, IL-2, PDGF, and nitric oxide (2). The up-regulation of FGF basic in many human tumors is associated with tumor vascularity (2, 3). Among FGF family members, only FGF acidic and FGF basic lack signal peptides and are secreted by an alternative pathway. FGF basic is additionally localized in the nucleus where it regulates gene expression (2, 7). In humans, the use of alternative start sites can produce 21-23 kDa isoforms found only in the nucleus, and these isoforms target the expression of distinct genes (7). FGF family proteins share 35-60% amino acid (aa) identity. The 18 kDa canine FGF basic shares 97-99% amino acid identity with bovine, equine, human, mouse, and rat FGF basic (8). FGF basic binds with picomolar affinity to FGF R1c and 2c (1, 2). Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha v beta 3, soluble FGF R1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen,
alpha 2-Macroglobulin, PDGF, and CXCL4/PF4 (2). These molecules act as cellular co-receptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones (2). In particular, the interaction of FGF basic with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors (1, 2).

Item	Value
References	Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159. Grose, R. and C. Dickson (2005) Cytokine Growth Factor Rev. 16:179. Rosenblatt-Velin, N. et al. (2005) J. Clin. Invest. 115:1724. Reuss, B. et al. (2003) Cell Tissue Res. 313:139. Su, N. et al. (2008) Front. Biosci. 13:2842. Claus, P. et al. (2003) J. Biol. Chem. 278:479. Abraham, J.A. et al. (1986) Science 233:545.
Long Name	Fibroblast Growth Factor basic
Entrez Gene IDs	2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names	Basic fibroblast growth factor, FGF basic, FGF-2, FGF2, FGFBprostatropin, HBGF-2, Prostatropin, bFGF, basic fibroblast growth factor bFGF, fibroblast growth factor 2 (basic), heparin-binding growth factor 2

Citations for Recombinant Canine FGF basic/FGF2/bFGF Protein (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Canine FGF basic/FGF2/bFGF Protein include:

Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

Ozelo M et al. (2014), Omental implantation of BOECs in hemophilia dogs results in circulating FVIII antigen and a complex immune response. Blood, 123(26):4045-53.
PMID: 24829206

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FAQs for Recombinant Canine FGF basic/FGF2/bFGF Protein

What receptors does FGF basic bind?

FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.bio-techne.com/resources/articles/fibroblast-growth-factors-and-their-receptors

Recombinant Canine FGF basic/FGF2/bFGF Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Canine FGF basic/FGF2/bFGF Protein

Formulation, Preparation and Storage

Carrier Free

8575-FB

Carrier

8575-FB/CF

Carrier Free

Reconstitution Calculator

Background: FGF basic/FGF2/bFGF

Citations for Recombinant Canine FGF basic/FGF2/bFGF Protein (1)

FAQs for Recombinant Canine FGF basic/FGF2/bFGF Protein

Product Documents for Recombinant Canine FGF basic/FGF2/bFGF Protein

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