Recombinant Bovine VEGF 164 Protein

Name: Recombinant Bovine VEGF 164 Protein
Brand: R&D Systems
SKU: 8917-BV
Price: 548 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 8917-BV

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Key Product Details

Source:	CHO
Accession #:	P15691
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Bovine VEGF 164 Protein

Source	Chinese Hamster Ovary cell line, CHO-derived bovine VEGF protein Ala27-Arg190
Purity	>95%, by SDS-PAGE with silver staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala27
Predicted Molecular Mass	19 kDa
SDS-PAGE	21-26 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using bAEC bovine aortic endothelial cells. The ED₅₀ for this effect is 0.3-1.8 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

8917-BV

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8917-BV/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: VEGF

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1, 2). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure (3). Humans express two sets of alternatively spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (3, 4). Isoforms other than VEGF₁₂₁ contain basic heparin-binding regions and are not freely diffusible (3, 4). VEGF₁₆₅ appears to be the most abundant and potent of the angiogenic isoform set, followed by VEGF₁₂₁ and VEGF₁₈₉ (3, 5). The anti-angiogenic or “b” set of isoforms is differentially spliced to contain five alternative amino acids at the C-terminus, and are the more highly expressed isoforms in normal adult tissue (6). VEGF_165b, like VEGF₁₂₁ but unlike most angiogenic isoforms, does not bind heparins and is therefore diffusible (3). Mature bovine VEGF corresponds to the VEGF₁₆₅ isoform of human VEGF. It shares 93%-97% aa sequence identity with common regions of human, porcine, equine, canine, and feline VEGF, and 89% with mouse and rat VEGF. In addition to alternatively spliced VEGF isoforms, multiple fragments of VEGF can be generated by extracellular proteolysis (4). VEGFs bind the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (3). Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 5). Human VEGF₁₆₅ binds the semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2 (7). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 5). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (5). Pathologically, it is involved in tumor development and tumor vascular leakage (8). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, and systemic lupus erythematosus (9). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M, and TNF-alpha (5, 10).

Item	Value
References	Leung, D.W. et al. (1989) Science 246:1306. Keck, P.J. et al. (1989) Science 246:1309. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853. Vempati, P. et al. (2014) Cytokine Growth Factor Rev. 25:1. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777. Nowak, D.G. et al. (2008) J. Cell Sci. 121:3487. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049. Goel, H.L. and A.M. Mercurio (2013) Nat. Rev. Cancer 13:871. Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246. Angelo, L.S. and R. Kurzrock (2007) Clin. Cancer Res. 13:2825.
Long Name	Vascular Endothelial Growth Factor
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names	MVCD1, VAS, VEGF, VEGF-A, VEGFA, VEGFMGC70609, VPF, VPFvascular endothelial growth factor, Vascular permeability factor, Vasculotropin, vascular endothelial growth factor A

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Recombinant Bovine VEGF 164 Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Bovine VEGF 164 Protein

Formulation, Preparation and Storage

Carrier Free

8917-BV

Carrier

8917-BV/CF

Carrier Free

Reconstitution Calculator

Background: VEGF

Citations for Recombinant Bovine VEGF 164 Protein

Product Documents for Recombinant Bovine VEGF 164 Protein

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