Recombinant A. caninum NIF Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5845-NF
Key Product Details
- R&D Systems NS0-derived Recombinant A. caninum NIF Protein (5845-NF)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Source
Mouse myeloma cell line, NS0-derived a. caninum NIF protein
Asn18-Leu274, with a C-terminal 6-His tag
Asn18-Leu274, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asn18
Predicted Molecular Mass
30 kDa
SDS-PAGE
55-65 kDa, reducing conditions
Activity
Measured by its ability to inhibit the adhesion of PMA-activated human neutrophils.
The ED50 for this effect is 0.1-0.4 μg/mL.
Also measured by its ability to bind Recombinant Human Integrin alphaM beta2 (Catalog # 4047-AM) in a functional ELISA.
The ED50 for this effect is 0.1-0.4 μg/mL.
Also measured by its ability to bind Recombinant Human Integrin alphaM beta2 (Catalog # 4047-AM) in a functional ELISA.
Reviewed Applications
Read 1 review rated 5 using 5845-NF in the following applications:
Formulation, Preparation and Storage
5845-NF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, and DTT. |
| Reconstitution |
Reconstitute at 200 μg/mL in PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: NIF
Neutrophil inhibitory factor (NIF) of the dog hookworm, Ancylostoma caninum, is a 41 kDa secreted glycoprotein that is thought to allow the hookworm to evade the innate immune defenses of the host (1, 2). NIF interacts with the leukocyte integrin alphaM beta2 (CD11b/CD18, also called Mac-1), thus inhibiting mammalian neutrophil adhesion to endothelium (1). The A. caninum NIF cDNA encodes 257 amino acids (aa) including a 17 aa signal sequence and a 240 aa mature protein with 10 cysteine residues and 7 potential N-linked glycosylation sites (1). Incubation of mammalian neutrophils with NIF does not appear to be toxic, but does result in rapid, cation‑dependent, reversible inhibition of alphaM beta2-mediated adhesion and degranulation (1 - 4). The NIF binding site within the alphaM beta2 I-domain interferes with activation‑dependent adhesion sites of ligands including ICAM-1/CD54, complement component C3b, and fibrinogen (2, 3). In mouse or guinea pig inflammatory lung injury models, administration of NIF prevented neutrophil-dependent vascular injury (4, 5).
References
- Moyle, M. et al. (1994) J. Biol. Chem. 269:10008.
- Rieu, P. et al. (1994) J. Cell Biol. 127:2081.
- Ustinov, V.A. and E.F. Plow (2002) J. Biol. Chem. 277:18769.
- Barnard, J.W. et al. (1995) J. Immunol. 155:4876.
- Zhou, M.-Y. et al. (1998) J. Clin. Invest. 101:2427.
Long Name
Neutrophil Inhibitory Factor
UniProt
Additional NIF Products
Product Documents for Recombinant A. caninum NIF Protein, CF
Product Specific Notices for Recombinant A. caninum NIF Protein, CF
For research use only
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