Recombinant Human RDH12 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78870

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NBP1-78870-50ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all RDH12 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 39-316 of Human RDH12

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGKVVV ITGANTGIGK ETARELASRG ARVYIACRDV LKGESAASEI RVDTKNSQVL VRKLDLSDTK SIRAFAEGFL AEEKQLHILI NNAGVMMCPY SKTADGFETH LGVNHLGHFL LTYLLLERLK VSAPARVVNV SSVAHHIGKI PFHDLQSEKR YSRGFAYCHS KLANVLFTRE LAKRLQGTGV TTYAVHPGVV RSELVRHSSL LCLLWRLFSP FVKTAREGAQ TSLHCALAEG LEPLSGKYFS DCKRTWVSPR ARNNKTAERL WNVSCELLGI RWE

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

33.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RDH12 His Protein

SDS-PAGE: Recombinant Human RDH12 His Protein [NBP1-78870]

SDS-PAGE: Recombinant Human RDH12 His Protein [NBP1-78870]

SDS-Page: Recombinant Human RDH12 His Protein [NBP1-78870] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-78870
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 40% glycerol, 0.2 M NaCl
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RDH12

Retinol dehydrogenase 12, also known as RDH12, belongs to the short-chain dehydrogenases /reductases (SDR) family. RDH12 is expressed, mostly in eye, kidney, brain, skeletal muscle and stomach. It is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. RDH12 also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Recombinant human RDH12 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

All-trans and 9-cis retinol dehydrogenase, EC 1.1.1, EC 1.1.1.-, EC 1.1.1.100, FLJ30273, LCA13retinol dehydrogenase 12, all-trans and 9-cis, LCA3, retinol dehydrogenase 12, retinol dehydrogenase 12 (all-trans and 9-cis), retinol dehydrogenase 12 (all-trans/9-cis/11-cis), SDR7C2, short chain dehydrogenase/reductase family 7C, member 2

Gene Symbol

RDH12

Additional RDH12 Products

Product Documents for Recombinant Human RDH12 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human RDH12 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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