If Ran is not in its GTP form, how to get Ran-GTP? (buffer, reaction time and temperature, GTP kind, etc)

We do not anticipate that this protein is active, and it is not recommend for functional assay. NBP1-45255 has been validated in ELISA (for standard curve) and PAGE (protein control) only. If wanting to perform functional assay, the mammalian cell expressed Ran protein will be more suitable. Unfortunately I do not have such information, but can only provide you the general references for you to check: Woo IS., et al. (2008) Apoptosis. 13(10):1223-31 and Ren M., et al. (1994) Mol Cell biol. 14(6):4216-24.

Is this protein good for RNA nuclear export assay?

Since we do not provide any active Ran, we do not have recommended protocol for RNA nuclear export assay.

Recombinant Human Ran His Protein

Name: Recombinant Human Ran His Protein
Brand: Novus Biologicals
SKU: NBP1-45255

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45255

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

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Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-216 of Human Ran

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

26.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Ran His Protein

SDS-PAGE: Recombinant Human Ran His Protein [NBP1-45255]

SDS-Page: Recombinant Human Ran Protein [NBP1-45255] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-45255

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ran

RAN, member RAS oncogene family, also known as RAN, is a small GTPase of the Ras superfamily that controls nucleocytoplasmic transport. It is involved in the directionality of the process that is regulated by GTP hydrolysis. Ran shuttles between the nucleus and the cytoplasm. Unlike many other regulatory GTPases, Ran is not posttranslationally modified so that it can be targeted to the subcellular compartment where it acts. Rather, RanGTP has been thought to be localized by the differential localization of Ran regulators. Recombinant human RAN, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Alternate Names

Androgen receptor-associated protein 24, ARA24Gsp1, GTPase Ran, GTP-binding nuclear protein Ran, guanosine triphosphatase Ran, member RAS oncogene family, OK/SW-cl.81, RAN, member RAS oncogene family, RanGTPase, Ras-like protein TC4, Ras-related nuclear protein, TC4

Gene Symbol

RAN

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Product Documents for Recombinant Human Ran His Protein

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COA

Product Specific Notices for Recombinant Human Ran His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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