Recombinant Human PU.1/Spi-1 His Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-51976
Key Product Details
Source
E. coli
Tag
His
Conjugate
Unconjugated
Applications
SDS-PAGE
Product Specifications
Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-271 of Human PU.1/Spi-1
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLQACKM EGFPLVPPQP SEDLVPYDTD LYQRQTHEYY PYLSSDGESH SDHYWDFHPH HVHSEFESFA ENNFTELQSV QPPQLQQLYR HMELEQMHVL DTPMVPPHPS LGHQVSYLPR MCLQYPSLSP AQPSSDEEEG ERQSPPLEVS DGEADGLEPG PGLLPGETGS KKKIRLYQFL LDLLRSGDMK DSIWWVDKDK GTFQFSSKHK EALAHRWGIQ KGNRKKMTYQ KMARALRNYG KTGEVKKVKK KLTYQFSGEV LGRGGLAERR HPPH
Purity
>85%, by SDS-PAGE
Predicted Molecular Mass
33.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Application Notes
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human PU.1/Spi-1 His Protein
SDS-PAGE: Recombinant Human PU.1/Spi-1 His Protein [NBP2-51976]
SDS-Page: PU.1/Spi-1 Recombinant Protein [NBP2-51976] - 15% SDS Page (3 ug)Formulation, Preparation and Storage
NBP2-51976
| Formulation | 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol |
| Preservative | No Preservative |
| Concentration | 0.25 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: PU.1/Spi-1
Long Name
Hematopoietic transcription factor PU.1
Alternate Names
OF, SFPI1, Spi-1, SPI-A, SPI1
Gene Symbol
SPI1
Additional PU.1/Spi-1 Products
Product Documents for Recombinant Human PU.1/Spi-1 His Protein
Product Specific Notices for Recombinant Human PU.1/Spi-1 His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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