Recombinant Protein L Type 1 His (N-Term) Cys (C-Term) Protein

Name: Recombinant Protein L Type 1 His (N-Term) Cys (C-Term) Protein
Brand: Novus Biologicals
SKU: NBP2-35050

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35050

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His (N-Term), Cys (C-Term)

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Protein L Type 1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 373 amino acids corresponding to Protein L Type 1 with a His tag on the N-terminus and a Cys on the C-terminus. However it migrates with an apparent molecular mass of 46 kDa in SDS-PAGE Source: E. coli

Uniprot ID: D6S9W1

Amino Acid Sequence: MHHHHHHKEE TPETPETDSE EEVTIKANLI FANGSTQTAE FKGTFEKATS EAYAYADTLK KDNGEYTVDV ADKGYTLNIK FAGKEKTPEE PKEEVTIKAN LIYADGKTQT AEFKGTFEEA TAEAYRYADA LKKDNGEYTV DVADKGYTLN IKFAGKEKTP EEPKEEVTIK ANLIYADGKT QTAEFKGTFE EATAEAYRYA DLLAKENGKY TVDVADKGYT LNIKFAGKEK TPEEPKEEVT IKANLIYADG KTQTAEFKGT FAEATAEAYR YADLLAKENG KYTADLEDGG YTINIRFAGK KVDEKPEEKE QVTIKENIYF EDGTVQTATF KGTFAEATAE AYRYADLLSK EHGKYTADLE DGGYTINIRF AGC

Purity

>95%, by SDS-PAGE

Endotoxin Level

Less than 0.1 EU/ug of Protein L Type 1 as determined by LAL method.

Predicted Molecular Mass

41.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Protein L is a genetically engineered protein containing 5 IgG-binding regions of protein L. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein L to ensure the maximum specific IgG binding. Protein L binds to human, mouse, rat and pig IgG.

Specificity

Protein L binds to human, mouse, rat and pig IgG.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Protein L Type 1 His (N-Term) Cys (C-Term) Protein

SDS-PAGE: Recombinant Protein L Type 1 His (N-Term) Cys (C-Term) Protein [NBP2-35050]

SDS-Page: Protein L Type 1 Cys (C-Term), His (N-Term) Protein [NBP2-35050]

Protein L Type 1 Cys (C-Term), His (N-Term) Protein [NBP2-35050]

Formulation, Preparation and Storage

NBP2-35050

Formulation	Lyophilized from additive free solution.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Reconstitute with distilled water or saline.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Protein L Type 1

Protein L contains 5 kappa-binding domain. Protein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins.

Additional Protein L Type 1 Products

Product Documents for Recombinant Protein L Type 1 His (N-Term) Cys (C-Term) Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Protein L Type 1 His (N-Term) Cys (C-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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