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Recombinant Protein A/G Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34862

Novus Biologicals, part of Bio-Techne
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NBP2-34862

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Functional Assay, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 430 amino acids corresponding to Protein A/G Source: E. coli

Amino Acid Sequence: CNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE GSGSGTYKLI LNGKTLKGET TTEAVDAATA EKVFKQYAND NGVDGEWTYD DATKTFTVTE KPEVIDASEL TPAVTTYKLV INGKTLKGET TTKAVDAETA EKAFKQYAND NGVDGVWTYD DATKTFTVTE

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Protein A/G as determined by LAL method.

Predicted Molecular Mass

47.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Protein A/G is a genetically engineered protein containing 5 IgG-binding regions of protein A and 2 of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the Protein A/G to ensure the maximum specific IgG binding. Protein A/G is ideal for purification of polyclonal or monoclonal IgG antibodies. It binds to various human, mouse and rat IgG subclasses and to total IgG from cow, goat, sheep, house, rabbit, guinea pig, pig, dog and cat.

Specificity

Recombinant Protein A/G binds to various human, mouse and rat IgG subclasses (e.g. human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c) and to total IgG from cow, goat, sheep, house, rabbit, guinea pig, pig, dog and cat.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-34862
Formulation Lyophilized from additive free solution.
Preservative No Preservative
Concentration Lyoph
Reconstitution Dissolve in distilled water or saline.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Protein A/G

Protein A/G consists of 7 IgG-binding domains EDABC-C1C3, which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C1 and C3. The binding capacity of recombinant Protein A/G is broader than either Protein A or Protein G alone.

Additional Protein A/G Products

Product Documents for Recombinant Protein A/G Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Protein A/G Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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