Recombinant Protein A/G/L Cys (N-Term) Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34961
Key Product Details
Source
E. coli
Tag
Cys (N-Term)
Conjugate
Unconjugated
Applications
Bioactivity, SDS-PAGE
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 806 amino acids corresponding to Protein A/G/L with a Cys on the N-terminusSource: E. coli
Amino Acid Sequence: CNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE GSGSGTYKLI LNGKTLKGET TTEAVDAATA EKVFKQYAND NGVDGEWTYD DATKTFTVTE KPEVIDASEL TPAVTTYKLV INGKTLKGET TTKAVDAETA EKAFKQYAND NGVDGVWTYD DATKTFTVTE EPRARPGSGS GKEETPETPE TDSEEEVTIK ANLIFANGST QTAEFKGTFE KATSEAYAYA DTLKKDNGEY TVDVADKGYT LNIKFAGKEK TPEEPKEEVT IKANLIYADG KTQTAEFKGT FEEATAEAYR YADALKKDNG EYTVDVADKG YTLNIKFAGK EKTPEEPKEE VTIKANLIYA DGKTQTAEFK GTFEEATAEA YRYADLLAKE NGKYTVDVAD KGYTLNIKFA GKEKTPEEPK EEVTIKANLI YADGKTQTAE FKGTFAEATA EAYRYADLLA KENGKYTADL EDGGYTINIR FAGKKVDEKP EEKEQVTIKE NIYFEDGTVQ TATFKGTFAE ATAEAYRYAD LLSKEHGKYT ADLEDGGYTI NIRFAG
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of Protein A/G/L as determined by LAL method.
Predicted Molecular Mass
89.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Protein A/G/L is a genetically engineered protein containing 5 IgG-binding regions of protein A, 2 of protein G and 5 of Protein L. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A/G/L to ensure the maximum specific IgG binding. The recombinant Protein A/G/L is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A/G/L binds to human, mouse, rat, cow, goat, sheep, rabbit, guinea pig, pig, dog and cat IgG.
Specificity
Protein A/G/L binds to human, mouse, rat cow, goat, sheep, rabbit, guinea pig, pig, dog and cat IgG.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-34961
| Formulation | Lyophilized from additive free solution. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Dissolve in distilled water or saline. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Protein A/G/L
Additional Protein A/G/L Products
Product Documents for Recombinant Protein A/G/L Cys (N-Term) Protein
Product Specific Notices for Recombinant Protein A/G/L Cys (N-Term) Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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