Recombinant Human Proteasome 20S beta2 His Protein

Name: Recombinant Human Proteasome 20S beta2 His Protein
Brand: Novus Biologicals
SKU: NBP1-78820

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78820

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Proteasome 20S beta2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-201 of Human Proteasome 20S beta2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLAD CLRSRTPYHV NLLLAGYDEH EGPALYYMDY LAALAKAPFA AHGYGAFLTL SILDRYYTPT ISRERAVELL RKCLEELQKR FILNLPTFSV RIIDKNGIHD LDNISFPKQG S

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

24.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Proteasome 20S beta2 His Protein

SDS-PAGE: Recombinant Human Proteasome 20S beta2 His Protein [NBP1-78820]

SDS-Page: Recombinant Human Proteasome 20S beta2 Protein [NBP1-78820] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-78820

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 30% glycerol, 0.1 M NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Proteasome 20S beta2

Proteasome subunit beta type-2, also known as PSMB2, is a member of the proteasome T1B family, which is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. Recombinant human PSMB2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Proteasome subunit beta type-2

Alternate Names

PSMB2

Gene Symbol