Recombinant Human Proinsulin C-Peptide Analogue Protein

Name: Recombinant Human Proinsulin C-Peptide Analogue Protein
Brand: Novus Biologicals
SKU: NBP2-35211

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35211

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P01308

Conjugate

Unconjugated

Applications

Western Blot, SDS-PAGE

View all Proinsulin C-Peptide Analogue Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 35 amino acids corresponding to Proinsulin C-Peptide Analogue Source: E. coli

Uniprot ID: P01308

Amino Acid Sequence: RREAEDLQVG QVELGGGPGA GSLQPLALEG SLQKR

A single non-glycosylated polypeptide chain containing 35 amino acids corresponding to Proinsulin C-Peptide Analogue Source: E. coli

Uniprot ID: P01308

Amino Acid Sequence: RREAEDLQVG QVELGGGPGA GSLQPLALEG SLQKR

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Proinsulin C-Peptide Analogue as determined by LAL method.

Predicted Molecular Mass

3.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Use in WB reported in secitific publication PMID: 32433667

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Proinsulin C-Peptide Analogue Protein

SDS-PAGE: Recombinant Human Proinsulin C-Peptide Analogue Protein [NBP2-35211]

SDS-Page: Human Proinsulin C-Peptide Analogue Protein [NBP2-35211]

Formulation, Preparation and Storage

NBP2-35211

Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Proinsulin C-Peptide Analogue

Proinsulin, a molecular weight of 8 kDa to 10 kDa, is a precursor of insulin. It has minimum hormonal activity and is converted to insulin by removal of the connecting C-peptide. Mature insulin has 35 fewer amino acids; 4 are removed altogether and the remaining 31 form the C-peptide. The C-peptide is abstracted from the center of the proinsulin sequence; the two other ends (the B chain and A chain) remain connected by disulfide bonds. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle and glycogen synthesis in liver.

Gene Symbol

INS

UniProt